Transgenically produced prolactin

• US 6,545,198 B1
• Filed: 01/24/2000
• Issued: 04/08/2003
• Est. Priority Date: 06/17/1997
• Status: Expired due to Fees

• Current Assignee: GTC Biotherapeutics, Inc.
• Original Assignee: Genzyme Transgenics Corporation
• Inventors: Echelard, Yann, Wilburn, Brian
• Primary Examiner(s): Shukla, Ram R.
• Application Number:
  US09/490,657
• Time in Patent Office: 1,170 Days
• Field of Search: 800/8, 800/4, 800/21, 800/14, 800/7
• US Class Current: 800/4
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A23C 2230/05 Milk or milk products from transgenic animals
• CPC Class Codes: C07K 14/57554 Prolactin

Abstract

Transgenically produced prolactin and methods of making and using transgenically produced prolactin.

Claims

1. A method of making transgenic prolactin in the milk of a non-human transgenic mammal comprising:

providing a non-human transgenic mammal whose genome comprises a transgene, wherein said transgene comprises a polynucleotide encoding a transgenic prolactin protein operably linked to a mammary gland specific promoter;

allowing the transgene to be expressed in the mammary gland of the non-human transgenic mammal; and

obtaining milk from the non-human transgenic mammal, wherein the milk comprises transgenic prolactin molecules and wherein less than 20 percent of the transgenic prolactin molecules in the milk are glycosylated, thereby making transgenic prolactin.

2. The method of claim 1, wherein said transgenic prolactin is human prolactin.

3. The method of claim 1, wherein said non-human transgenic mammal is a transgenic goat.

4. A method of making heterologous prolactin in the milk of a non-human transgenic mammal, comprising:

obtaining milk from a non-human transgenic mammal whose genome comprises a transgene, wherein said transgene comprises a polynucleotide encoding a heterologous prolactin protein operably linked to a mammary gland specific promoter, wherein less than 20 percent of the prolactin molecules in the milk are glycosylated, thereby making heterologous prolactin.

5. The method of claim 4, wherein said heterologous prolactin is human prolactin.

6. A method of making transgenic prolactin in the milk of a non-human transgenic mammal comprising:

providing a non-human transgenic mammal whose genome comprises a transgene

wherein said transgene comprises a polynucleotide encoding a transgenic prolactin protein operably linked to a mammary gland specific promoter;

allowing the transgene to be expressed in the mammary gland of the non-human transgenic mammal; and

obtaining milk from the non-human transgenic mammal, wherein the milk comprises transgenic prolactin molecules and wherein less than 10 percent of the transgenic prolactin molecules in the milk are glycosylated, thereby making transgenic prolactin.

7. The method of claim 6, wherein less than 5 percent of the transgenic prolactin molecules in the milk of the non-human transgenic mammal are glycosylated.

8. The method of claim 7, wherein said transgenic prolactin is human prolactin.

9. A method of making heterologous prolactin in the milk of a non-human transgenic mammal, comprising:

obtaining milk from a non-human transgenic mammal whose genome comprises a transgene, wherein said transgene comprises a polynucleotide encoding a heterologous prolactin protein operably linked to a mammary gland specific promoter, wherein less than 10 percent of the prolactin molecules in the milk are glycosylated, thereby making heterologous prolactin.

10. The method of claim 9, wherein less than 5 percent of the heterologous prolactin molecules in the milk of the non-human transgenic mammal are glycosylated.

11. The method of claim 9, wherein said heterologous prolactin is human prolactin.