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Biosensor and method for quantitating biochemical substrate using the same

  • US 6,547,954 B2
  • Filed: 01/18/1999
  • Issued: 04/15/2003
  • Est. Priority Date: 03/12/1996
  • Status: Expired due to Term
First Claim
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1. A biosensor comprising:

  • an insulating substrate;

    an electrode system formed on the insulating substrate which comprises a working electrode and a counter electrode; and

    a solid phase reaction layer formed on the insulating substrate which contains a solid phase oxidoreductase and a solid phase ferrocene electrolyte as an electron acceptor, the solid phase reaction layer being formed over at least a portion of the working electrode or the counter electrode, wherein the ferrocene electrolyte consists of a ferricinium ion and a mono-anion, and wherein the ferricinium ion is provided in the biosensor together with the oxidoreductase upon dissolution of the reaction layer.

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