Surface-bound, double-stranded DNA protein arrays

• US 6,548,021 B1
• Filed: 08/11/1998
• Issued: 04/15/2003
• Est. Priority Date: 10/10/1997
• Status: Expired due to Term

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Bulyk, Martha L., Church, George M.
• Primary Examiner(s): Marschel, Ardin H.
• Application Number:
  US09/132,368
• Time in Patent Office: 1,708 Days
• Field of Search: 435/4-6, 435/7.1, 435/7.2, 436/501, 536/22.1, 536/25.3, 422/50, 422/68.1
• US Class Current: 422/68.1
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C07B 2200/11 Compounds covalently bound to a solid support
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2522/101 Single or double stranded nucleic acid binding proteins
• CPC Class Codes: C12Q 2563/107 fluorescence
• CPC Class Codes: C12Q 2565/525 characterised by the capture oligonucleotide being double stranded
• CPC Class Codes: C40B 40/00 Libraries per se, e.g. arrays, mixtures

Abstract

The invention provides a synthetic array of surface-bound, bimolecular, double-stranded nucleic acid molecules, the array comprising a solid support and a plurality of bimolecular double-stranded nucleic acid molecule members, a member comprising a first nucleic acid strand linked to the solid support and a second nucleic acid strand which is substantially complementary to the first strand and complexed to the first strand by Watson-Crick base pairing, wherein for at least a portion of the members, each member comprises a recognition site within a nucleic acid sequence for a protein, wherein a recognition site within a nucleic acid sequence for a protein of a first member is different from a recognition site within a nucleic acid sequence for a protein of a second member and wherein a protein is bound to a member thereof.

Claims

1. A synthetic array comprising:

a solid support, and a plurality of double-stranded nucleic acid molecules, wherein each nucleic acid molecule comprises a first nucleic acid strand linked to the solid support and a second nucleic acid strand which is substantially complementary to the first nucleic acid strand and hybridized to the first nucleic acid strand, wherein the 5′

end of the first nucleic acid strand and the 3′

end of the second nucleic acid strand are covalently unbound to each other, wherein each nucleic acid molecule comprises a protein recognition site within a double stranded nucleic acid sequence, wherein a protein recognition site within a first nucleic acid molecule is different from a protein recognition site within a second nucleic acid molecule, and a protein is bound to at least one of the nucleic acid molecules,

wherein each protein recognition site within a nucleic acid molecule is selected from the group that includes naturally-occurring protein recognition sites, synthetic variants of naturally-occurring protein recognition sites and randomized nucleic acid sequences,

and wherein at least one protein recognition site within a nucleic acid molecule comprises first and second half-sites, wherein the first half-site is recognized by a different protein than is the second half-site.

2. The array of claim 1, wherein the 3′

end of the first nucleic acid strand is linked to the solid support.

3. The array of claim 1, wherein the 5′

end of the second nucleic acid strand is not linked to the solid support.

4. The array of claim 1, wherein the bound protein comprises a detectable label.

5. The array of claim 1, wherein the bound protein is a chimeric protein.

6. The array of claim 5, wherein said chimeric protein comprises a DNA-binding domain fused in-frame with a protein:

protein dimerization domain.

7. The array of claim 5, wherein said chimeric protein comprises a DNA-binding domain fused in-frame to Green Fluorescent Protein.

8. The array of claim 1, wherein said solid support is a silica support.

9. The array of claim 1, wherein the first nucleic acid strand is used as the template on which the second nucleic acid strand is produced by enzymatic synthesis.

10. The array of claim 9, wherein the first nucleic acid strand comprises at its 3′

end a binding site for an oligonucleotide primer which is used to prime enzymatic synthesis of the second nucleic acid strand, and the first nucleic acid strand comprises at its 5′

end a variable sequence.

11. The array of claim 9, wherein said enzymatic synthesis is performed using an enzyme.

12. The array of claim 10, wherein said oligonucleotide primer is between 10 and 30 nucleotides in length.

13. The array of claim 1, wherein the first nucleic acid strand comprises DNA.

14. The array of claim 1, wherein the second nucleic acid strand comprises DNA.

15. The array of claim 1, wherein the first and second nucleic acid strands each comprise from 16 to 60 monomers selected from the group that include ribonucleotides and deoxyribonucleotides.

16. The array of claim 1, wherein the solid support is a silica support and the first and second nucleic acid strands each comprise from 16 to 60 monomers selected from the group that includes ribonucleotides and deoxyribonucleotides.

17. The array of claim 1, wherein at least a portion of said plurality have a second nucleic acid strand that is substantially complementary to- and based-paired with said first strand along the entire length of said first strand.

18. The array of claim 4, wherein the detectable label is radioactive, fluorescent, chemiluminescent, or chromogenic.

19. The array of claim 1, wherein the solid support has surface attaching groups.

20. The array of claim 19, wherein the surface attaching groups are selected from the group consisting of amine, hydroxyl, thiol, and carboxyl groups.

21. The array of claim 5, wherein the chimeric protein comprises a DNA-binding domain fused in frame with a protein:

protein dimerization domain.

22. The array of claim 5, wherein the chimeric protein comprises a DNA-binding domain fused in frame to Green Fluorescent Protein.