Microstructured bilateral sensor
First Claim
1. A microporous sensor for detecting an analyte comprising a substrate having a front face and a back face, and at least one pore extending through the substrate that interacts with the analyte, a membrane containing an enzyme disposed near the front face of the pore, an electrode, and a back membrane disposed at the back face of the pore, the back membrane being permeable to a co-substrate or other reagent.
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Accused Products
Abstract
A bilateral biosensor based upon a microporous architecture is provided which seeks to reduce the effect of co-reactant concentration limitations by utilizing a new sensor microgeometry. If implemented on the appropriate scale, the new sensor design augments substantially the concentration of oxygen, or other co-reactants or reagents, in the reaction zone of the sensor. Performance enhancements over traditional microscale devices employing unilateral orientation are accomplished, in one embodiment, by allowing analyte to enter the sensor from one side of the sensor, while allowing a co-substrate to enter from both sides of the sensor.
475 Citations
28 Claims
- 1. A microporous sensor for detecting an analyte comprising a substrate having a front face and a back face, and at least one pore extending through the substrate that interacts with the analyte, a membrane containing an enzyme disposed near the front face of the pore, an electrode, and a back membrane disposed at the back face of the pore, the back membrane being permeable to a co-substrate or other reagent.
- 20. A glucose sensor comprising a substrate having a front face and a back face and at least one pore extending through the substrate, a membrane containing an enzyme that reacts with glucose disposed near the front face of the pore, an electrode in electrochemical contact with the enzyme-containing membrane, and a back membrane that is permeable to oxygen such that oxygen can be supplied from the back face of the pore to the enzyme-containing membrane for reaction with glucose thereby providing the oxygen to the enzyme in quantities that improve sensor performance.
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28. A method for detecting an analyte which comprises:
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providing a microporous sensor including a substrate having a front face and a back face and at least one pore extending through the substrate, a membrane containing an enzyme disposed near the front face of the pore, an electrode and a back membrane disposed at the back face of the pore;
contacting a fluid suspected of containing an analyte with the front face of the substrate in the presence of a co-reactant for detecting the analyte; and
supplying said co-reactant from the back face of the substrate through the back membrane and the pore.
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Specification