Microstructured bilateral sensor

• US 6,551,496 B1
• Filed: 03/06/2001
• Issued: 04/22/2003
• Est. Priority Date: 03/03/2000
• Status: Active Grant

• Current Assignee: YSI Incorporated (Xylem, Inc.)
• Original Assignee: YSI Incorporated (Xylem, Inc.)
• Inventors: Madaras, Marcel, Leland, Quinn, Moles, Donald R.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/800,438
• Time in Patent Office: 777 Days
• Field of Search: 204/403.01, 204/403.06, 204/403.1, 204/403.11, 204/409, 205/778, 205/777.5
• US Class Current: 205/778
• CPC Class Codes: C12Q 1/001 Enzyme electrodes

Abstract

A bilateral biosensor based upon a microporous architecture is provided which seeks to reduce the effect of co-reactant concentration limitations by utilizing a new sensor microgeometry. If implemented on the appropriate scale, the new sensor design augments substantially the concentration of oxygen, or other co-reactants or reagents, in the reaction zone of the sensor. Performance enhancements over traditional microscale devices employing unilateral orientation are accomplished, in one embodiment, by allowing analyte to enter the sensor from one side of the sensor, while allowing a co-substrate to enter from both sides of the sensor.

Claims

1. A microporous sensor for detecting an analyte comprising a substrate having a front face and a back face, and at least one pore extending through the substrate that interacts with the analyte, a membrane containing an enzyme disposed near the front face of the pore, an electrode, and a back membrane disposed at the back face of the pore, the back membrane being permeable to a co-substrate or other reagent.

2. The sensor of claim 1 wherein the back membrane is able to supply sufficient co-substrate or other reagent to the pore to improve sensor performance.

3. The sensor of claim 1 wherein the enzyme is selected from the group consisting of glucose oxidases, galactose oxidases and uricases.

4. The sensor of claim 3 wherein the enzyme is useful in a glucose oxidase.

5. The sensor of claim 3 wherein the sensor additionally includes a barrier film interposed between the enzyme-containing membrane and the analyte.

6. The sensor of claim 5 wherein at least one pore is about 0.02 to 200 microns in diameter.

7. The sensor of claim 6 wherein the substrate is about 1 to 1,000 microns thick.

8. The sensor of claim 7 wherein the substrate is a film of a polymer selected from the group consisting of polyimide, polycarbonate and polyethylene terephthalate.

9. The sensor of claim 6 wherein the back membrane is polytetrafluroethylene or silicone rubber.

10. The sensor of claim 9 wherein the back membrane is silicone rubber.

11. The sensor of claim 10 wherein the barrier film is polyvinyl alcohol.

12. The sensor of claim 11 wherein the pores are in a random or ordered pattern.

13. The sensor of claim 12 wherein the pores are laser drilled.

14. The sensor of claim 6 wherein the substrate is a cyclotron irradiated film.

15. The sensor of claim 1 wherein the sensor includes a plurality of pores.

16. The sensor of claim 1 wherein the back membrane is permeable to a cosubstrate.

17. The sensor of claim 16 wherein the back membrane is permeable to oxygen.

18. The sensor of claim 1 wherein the electrode is formed on the wall of the pore.

19. The sensor of claim 1 wherein the electrode is formed at the perimeter of the pore on the back face of the substrate.

20. A glucose sensor comprising a substrate having a front face and a back face and at least one pore extending through the substrate, a membrane containing an enzyme that reacts with glucose disposed near the front face of the pore, an electrode in electrochemical contact with the enzyme-containing membrane, and a back membrane that is permeable to oxygen such that oxygen can be supplied from the back face of the pore to the enzyme-containing membrane for reaction with glucose thereby providing the oxygen to the enzyme in quantities that improve sensor performance.

21. The sensor of claim 20 wherein the enzyme is selected from the group consisting of glucose oxidases, galactose oxidases and uricases.

22. The sensor of claim 21 wherein the sensor includes a plurality of pores.

23. The sensor of claim 22 wherein the electrode is formed on the wall of the pore.

24. The sensor of claim 22 wherein the electrode is formed at the perimeter of the pore on the back face of the substrate.

25. The sensor of claim 20 wherein a barrier film of polyvinyl alcohol is provided over the enzyme-containing membrane on the front face of the pore.

26. The sensor of claim 25 wherein the pores are about 0.02 to 200 microns in diameter.

27. The sensor of claim 26 wherein the substrate is about 1 to 1,000 microns thick.

28. A method for detecting an analyte which comprises:

providing a microporous sensor including a substrate having a front face and a back face and at least one pore extending through the substrate, a membrane containing an enzyme disposed near the front face of the pore, an electrode and a back membrane disposed at the back face of the pore;

contacting a fluid suspected of containing an analyte with the front face of the substrate in the presence of a co-reactant for detecting the analyte; and

supplying said co-reactant from the back face of the substrate through the back membrane and the pore.