Nucleic acid and amino acid sequences relating to pseudomonas aeruginosa for diagnostics and therapeutics

• US 6,551,795 B1
• Filed: 02/18/1999
• Issued: 04/22/2003
• Est. Priority Date: 02/18/1998
• Status: Expired due to Fees

• Current Assignee: Genome Therapeutics Corporation
• Original Assignee: Genome Therapeutics Corporation
• Inventors: Rubenfield, Marc J., Deloughery, Craig, Nolling, Jork, Bush, David
• Primary Examiner(s): Allen, Marianne P.
• Application Number:
  US09/252,991
• Time in Patent Office: 1,524 Days
• Field of Search: 536/23.1, 536/23.7, 435/6, 435/320.1, 435/69.1, 435/253.3, 435/325, 424/184.1, 514/44
• US Class Current: 435/69.1
• CPC Class Codes: C07K 14/21 from Pseudomonadaceae (F)

Abstract

The invention provides isolated polypeptide and nucleic acid sequences derived from Pseudomonas aeruginosa that are useful in diagnosis and therapy of pathological conditions; antibodies against the polypeptides; and methods for the production of the polypeptides. The invention also provides methods for the detection, prevention and treatment of pathological conditions resulting from bacterial infection.

Claims

1. An isolated nucleic acid comprising a nucleotide sequence encoding a P. aeruginosa polypeptide selected from the group consisting of SEQ ID NO:

17411, SEQ ID NO;

17456, SEQ ID NO;

18830, SEQ ID NO;

21016, SEQ ID NO;

21380, SEQ ID NO;

22234, SEQ ID NO;

24200, SEQ ID NO;

27183, SEQ ID NO;

27280, and SEQ ID NO;

32329.

2. A recombinant expression vector comprising the nucleic acid of claim 1 operably linked to a transcription regulatory element.

3. A cell comprising a recombinant expression vector of claim 2.

4. A method for producing a P. aeruginosa polypeptide comprising culturing a cell of claim 3 under conditions that permit expression of the polypeptide.

5. An isolated nucleic acid comprising a nucleotide sequence encoding a P. aeruginosa polypeptide, said nucleic acid selected from the group consisting of SEQ ID NO:

840, SEQ ID NO;

885, SEQ ID NO;

2259, SEQ ID NO;

4445, SEQ ID NO;

4809, SEQ ID NO;

5663, SEQ ID NO;

7629, SEQ ID NO;

10612, SEQ ID NO;

10709, and SEQ ID NO;

15758.

6. A recombinant expression vector comprising the nucleic acid of claim 5 operably linked to a transcription regulatory element.

7. A cell comprising a recombinant expression vector of claim 6.

8. A method for producing a P. aeruginosa polypeptide comprising culturing a cell of claim 7 under conditions that permit expression of the polypeptide.

9. A probe comprising a nucleotide sequence of at least 30 nucleotides of a nucleotide sequence selected from the group consisting of SEQ ID NO:

4445, SEQ ID NO;

5663, SEQ ID NO;

10612, SEQ ID NO;

10709, and SEQ ID NO;

15758.

10. An isolated nucleic acid or the complement thereof comprising a nucleotide sequence of at least 40 nucleotides in length selected from the group consisting of SEQ ID NO:

4445, SEQ ID NO;

5663, SEQ ID NO;

10612, SEQ ID NO;

10709, and SEQ ID NO;

15758, wherein the sequence is hybridizable under conditions of high stringency to a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO;

4445, SEQ ID NO;

5663, SEQ ID NO;

10612, SEQ ID NO;

10709, and SEQ ID NO;

15758, or the complement thereof.

11. An isolated nucleic acid comprising a nucleotide sequence encoding a P. aeruginosa polypeptide selected from the group consisting of SEQ ID NO:

17456, SEQ ID NO;

18830, SEQ ID NO;

21016, SEQ ID NO;

21380, SEQ ID NO;

22234, SEQ ID NO;

27183, SEQ ID NO;

27280, and SEQ ID NO;

32329.

12. A recombinant expression vector comprising the nucleic acid of claim 11 operably linked to a transcription regulatory element.

13. A cell comprising a recombinant expression vector of claim 12.

14. A method for producing a P. aeruginosa polypeptide comprising culturing a cell of claim 13 under conditions that permit polypeptide expression.

15. An isolated nucleic acid comprising a nucleotide sequence with at least 80% sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NO:

885, SEQ ID NO;

2259, SEQ ID NO;

4445, SEQ ID NO;

4809, SEQ ID NO;

5663, SEQ ID NO;

10612, SEQ ID NO;

10709, and SEQ ID NO;

15758, and wherein said isolated nucleic acid encodes a P. aeruginosa polypeptide selected from the group consisting of SEQ ID NO;

17456, SEQ ID NO;

18830, SEQ ID NO;

21016, SEQ ID NO;

21380, SEQ ID NO;

22234, SEQ ID NO;

27183, SEQ ID NO;

27280, and SEQ ID NO;

32329, respectively.

16. A recombinant expression vector comprising the nucleic acid of claim 15 operably linked to a transcription regulatory element.

17. A cell comprising a recombinant expression vector of claim 16.

18. A method for producing a P. aeruginosa polypeptide comprising culturing a cell of claim 17 under conditions that permit polypeptide expression.

19. An isolated nucleic acid comprising a nucleotide sequence with at least 90% sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NO:

885, SEQ ID NO;

2259, SEQ ID NO;

4445, SEQ ID NO;

4809, SEQ ID NO;

5663, SEQ ID NO;

10612, SEQ ID NO;

10709, and SEQ ID NO;

15758 and wherein said isolated nucleic acid encodes a P. aeruginosa polypeptide selected from the group consisting of SEQ ID NO;

17456, SEQ ID NO;

18830, SEQ ID NO;

21016, SEQ ID NO;

21380, SEQ ID NO;

22234, SEQ ID NO;

27183, SEQ ID NO;

27280, and SEQ ID NO;

32329.

20. A recombinant expression vector comprising the nucleic acid of claim 19 operably linked to a transcription regulatory element.

21. A cell comprising a recombinant expression vector of claim 20.

22. A method for producing a P. aeruginosa polypeptide comprising culturing a cell of claim 21 under conditions that permit polypeptide expression.

23. An isolated nucleic acid comprising a nucleotide sequence encoding a P. aeruginosa polypeptide, said nucleic acid selected from the group consisting of SEQ ID NO:

885, SEQ ID NO;

2259, SEQ ID NO;

4445, SEQ ID NO;

4809, SEQ ID NO;

5663, SEQ ID NO;

10612, SEQ ID NO;

10709, and SEQ ID NO;

15758.

24. A recombinant expression vector comprising the nucleic acid of claim 23 operably linked to a transcription regulatory element.

25. A cell comprising a recombinant expression vector of claim 23.

26. A method for producing a P. aeruginosa polypeptide comprising culturing a cell of claim 25 under conditions that permit polypeptide expression.