Process for stabilizing the content of glycated protein of a sample on a matrix material

• US 6,552,165 B1
• Filed: 05/21/1999
• Issued: 04/22/2003
• Est. Priority Date: 08/24/1995
• Status: Expired due to Term

• Current Assignee: Roche Diagnostics GmbH (Roche Holding AG)
• Original Assignee: Roche Diagnostics GmbH (Roche Holding AG)
• Inventors: Nagel, Rolf, Mistele, Jürgen
• Primary Examiner(s): Brumback, Brenda
• Assistant Examiner(s): Gupta, Anish
• Application Number:
  US09/316,867
• Time in Patent Office: 1,432 Days
• Field of Search: 514/8, 530/322, 530/350
• US Class Current: 530/322
• CPC Class Codes: G01N 33/68 involving proteins, peptides or amino acids involving lipoproteins G01N33/92
• CPC Class Codes: G01N 33/6842 Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
• CPC Class Codes: G01N 33/723 Glycosylated haemoglobin
• CPC Class Codes: G01N 33/726 Devices

Abstract

The invention concerns a method for stabilizing the content of glycated protein in a sample on a matrix material, which is characterized in that the matrix material is impregnated with boric acid buffer with a pH which is larger or equal to 10.5 or a transition metal salt as well as an appropriate matrix material, an element for collecting, transporting and storing sample material to be analyzed with such a matrix material and a system containing such an element and a sealable covering.

Claims

1. A matrix material comprising a fibrous material which has been impregnated with a boric acid buffer at a pH of at least 10.5, or which carries a transition metal salt and which has a glycated protein absorbed thereon.

2. The matrix material of claim 1, wherein said water soluble transition metal salt is a nickel salt or a copper salt.

3. The matrix material of claim 2, wherein said water soluble transition metal salt is a nickel salt.

4. The matrix material of claim 2, wherein said water soluble transition metal salt is a copper salt.

5. A dried matrix material having impregnated thereon a water soluble transition metal salt and a glycated protein.

6. The matrix material of claim 5, wherein said water soluble transition metal salt is a nickel salt or a copper salt.

7. The matrix material of claim 6, wherein said water soluble transition metal salt is a nickel salt.

8. The matrix material of claim 6, wherein said water soluble transition metal salt is a copper salt.