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Production of anti-self antibodies from antibody segment repertoires and displayed on phage

  • US 6,555,313 B1
  • Filed: 05/16/2000
  • Issued: 04/29/2003
  • Est. Priority Date: 12/02/1991
  • Status: Expired due to Fees
First Claim
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1. A method of obtaining a member of a specific binding pair, the specific binding pair member being an antibody or antibody fragment and having an antigen binding site comprising an antibody light chain variable region and an antibody heavy chain variable region, the antigen binding site having binding specificity for an antigen which is a human self antigen, the method comprising:

  • (a) providing a library of filamentous bacteriophage, each filamentous bacteriophage displaying at its surface a specific binding pair member, and each filamentous bacteriophage containing nucleic acid with sequence derived from human species unimmunized with said human self antigen and encoding a polypeptide chain which is a component part of the specific binding pair member displayed at the surface of that filamentous bacteriophage; and

    (b) selecting, by binding with said human self antigen, one or more specific binding pair members with binding specificity for said human self antigen.

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