Xylanase from Trichoderma reesei , method for production thereof, and methods employing this enzyme

• US 6,555,335 B1
• Filed: 09/11/2000
• Issued: 04/29/2003
• Est. Priority Date: 12/30/1999
• Status: Expired due to Term

• Current Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Original Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Inventors: Siika-Aho, Matti, Penttila, Merja E., Tenkanen, Maija, Saloheimo, Markku La
• Primary Examiner(s): Yucel, Remy
• Assistant Examiner(s): DAVIS, KATHARINE F
• Application Number:
  US09/658,772
• Time in Patent Office: 960 Days
• Field of Search: 435/183, 435/200, 435/209, 435/220, 435/223, 435/225, 435/195, 435/471, 435/252.31, 435/253.33, 435/254.6, 435/41
• US Class Current: 435/41
• CPC Class Codes: C12N 9/248 Xylanases

Abstract

The present invention is directed to novel xylanases (referred to as XYL-IV) and to nucleic acid molecules encoding those xylanases. Also provided herein are vectors and host cells including those nucleic acid sequences, antibodies which bind to the xylanases of the present invention, methods for producing the xylanases of the present invention, and methods employing the xylanases of the present invention.

Claims

1. An isolated xylanase (XYL-IV) comprising the amino acid sequence of SEQ ID NO:

2.

2. The isolated XYL-IV of claim 1, wherein the XYL-IV is derived from a filamentous fungus.

3. The isolated XYL-IV of claim 2, wherein the filamentous fungus is a Trichoderma spp.

4. A method of altering the properties of a xylan containing substrate comprising

(a) obtaining a host cell which has been transformed with a vector comprising DNA encoding a xylanase (XYL-IV) having the amino acid sequence of SEQ ID NO:

2;

(b) culturing the host cell under conditions suitable for the expression of the XYL-IV;

(c) recovering the XYL-IV; and

(d) contacting the xylan containing substrate with a composition including the XYL-IV.

5. The method according to claim 4, wherein the host cell is a filamentous fungal cell.

6. The method according to claim 4, wherein the host cell is from a strain of Bacillus or a strain of Escherichia coli.

7. The method of claim 4, wherein said method comprises altering the handling properties of animal feed and food products.

8. The method of claim 4, wherein said method comprises altering plant materials.

9. The method of claim 4, wherein said method comprises altering the properties of wood pulp or derivatives thereof during the manufacture of paper.

10. The method of claim 4, wherein said method comprises altering the properties of xylan containing biomass during its reduction to glucose.