Methods for amplifying and sequencing nucleic acid molecules using a three component polymerase
First Claim
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1. A method for amplifying a nucleic acid molecule comprising:
- subjecting said nucleic acid molecule to a polymerase chain reaction process utilizing a three component DNA polymerase, wherein the three component DNA polymerase comprises a DNA polymerase component, a sliding clamp component, and a clamp loader component.
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Abstract
The present invention is directed to a method for amplifying or sequencing a nucleic acid molecule with a three component polymerase comprising a DNA polymerase component, a sliding clamp component, and a clamp loader component.
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Citations
42 Claims
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1. A method for amplifying a nucleic acid molecule comprising:
subjecting said nucleic acid molecule to a polymerase chain reaction process utilizing a three component DNA polymerase, wherein the three component DNA polymerase comprises a DNA polymerase component, a sliding clamp component, and a clamp loader component. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method for dideoxy sequencing a nucleic acid molecule comprising:
subjecting said nucleic acid molecule to a nucleic acid sequencing process with a three component DNA polymerase, wherein the three component DNA polymerase comprises a DNA polymerase component, a sliding clamp component, and a clamp loader component. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
- 24. A kit for amplifying a nucleic acid molecule comprising a three component DNA polymerase comprising a DNA polymerase component, a sliding clamp component, and a clamp loader component and at least one deoxynucleoside triphosphate.
- 33. A kit for sequencing a nucleic acid molecule comprising a carrier means having in close confinement therein two or more container means, wherein a first container means contains a three component DNA polymerase comprising a DNA polymerase component, a sliding clamp component, and a clamp loader component and a second container means contains at least one dideoxynucleoside triphosphate.
Specification