Multiple mesodermal lineage differentiation potentials for adipose tissue-derived stromal cells and uses thereof

• US 6,555,374 B1
• Filed: 08/14/2000
• Issued: 04/29/2003
• Est. Priority Date: 08/19/1999
• Status: Expired due to Term

• Current Assignee: Cognate BioServices, Inc.
• Original Assignee: Artecel Sciences Inc.
• Inventors: Gimble, Jeffrey Martin, Wilkison, William O., Halvorsen, Juan-Di Chang
• Primary Examiner(s): Weber, Jon P.
• Application Number:
  US09/638,544
• Time in Patent Office: 988 Days
• Field of Search: 435/366, 435/370, 435/371, 435/372
• US Class Current: 435/371
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: C12N 5/0647 Haematopoietic stem cells; Uncommitted or multipotent progenitors
• CPC Class Codes: C12N 5/0667 Adipose-derived stem cells [ADSC]; Adipose stromal stem cells

Abstract

The invention relates to methods and compositions for the differentiation of stromal cells from adipose tissue into hematopoietic supporting stromal cells and myocytes of both the skeletal and smooth muscle type. The cells produced by the methods are useful in providing a source of fully differentiated and functional cells for research, transplantation and development of tissue engineering products for the treatment of human diseases and traumatic tissue injury repair.

Claims

1. A non-naturally-occurring mixture of cells comprising an isolated extramedullary adipose tissue-derived stromal cell and a non-adipose derived cell capable of forming a blood cell.

2. The mixture of cells of claim 1, wherein the non-adipose-derived cell capable of forming a blood cell is selected from the group consisting of a hematopoietic stem cell and a hematopoietic progenitor cell.

3. The mixture of cells of claim 1, wherein the non-adipose-derived cells capable of forming a blood cell is a hematopoietic stem cell.

4. The mixture of cells of claim 1, wherein the non-adipose-derived cell capable of forming a blood cell is selected from the group consisting of a hematopoietic stem cell and a myeloid cell.

5. The mixture of cells of claim 1, wherein the non-adipose-derived cell capable of forming a blood cell is selected from the group consisting of a hematopoietic stem cell and a lymphoid cell.

6. The mixture of cells of claim 1, wherein the non-adipose-derived cell capable of forming a blood cell is selected from the group consisting of a hematopoietic stem cell and an erythroid cell.

7. The mixture of cells of claim 1, wherein the non-adipose-derived cell capable of forming a blood cell is selected from the group consisting of a hematopoietic stem cell and a megakaryocyte cell.

8. The mixture of cells of claim 1, wherein the non-adipose-derived cell capable of forming a blood cell is selected from the group consisting of a hematopoietic stem cell and an osteoclast.

9. The mixture of cells of claim 1, wherein the formed blood cell is a hematopoietic progenitor cell.

10. The mixture of cells of claim 1, wherein the formed blood cell is a hematopoietic stem cell.

11. The mixture of cells of claim 1, wherein the formed blood cell is a myeloid cell.

12. The mixture of cells of claim 1, wherein the formed blood cell is a lymphoid cell.

13. The mixture of cells of claim 1, wherein the formed blood cell is an erythroid cell.

14. The mixture of cells of claim 1, wherein the formed blood cell is a megakaryocyte cell.

15. The mixture of cells of claim 1, wherein the formed blood cell is an osteoclast.

16. The mixture of cells of claim 1, wherein the non-adipose-derived cell capable of forming a blood cell is selected from the group consisting of a hematopoietic progenitor cell, hematopoietic stem cell, myeloid cell, lymphoid cell, erythroid cell, megakaryocyte cell, and osteoclast.

17. The mixture of cells of claim 1, wherein the non-adipose-derived cell capable of forming a blood cell is selected from the group consisting of a hematopoietic a progenitor cell, hematopoietic stem cell and myeloid cell.

18. The mixture of cells of claim 1, wherein the non-adipose-derived cell capable of forming a blood cell is selected from the groups consisting of a hematopoietic stem cell, myeloid cell and lymphoid cell.

19. The mixture of cells of claim 1, wherein the non-adipose-derived cell capable of forming a blood cell is selected from the group consisting of a hematopoietic stem cell, erythroid cell and megakaryocyte cell.

20. The mixture of cells of claim 1, wherein the non-adipose-derived cell capable of forming a blood cell is selected from the group consisting of a hematopoietic stem cell, lymphoid cell and osteoclast.

21. The mixture of cells of claim 1, wherein the non-adipose-derived cell capable of forming a blood cell is selected from the group consisting of a hematopoietic stem cell, lymphoid cell and a megakaryocyte cell.

22. The mixture of cells of claim 1, wherein the non-adipose-derived cell capable of forming a blood cell is selected from the group consisting of a hematopoietic stem cell, erythroid cell and lymphoid cell.

23. The mixture of cells of claim 1, wherein the non-adipose-derived cell capable of forming a blood cell is selected from the group consisting of a hematopoietic stem cell, erythroid cell and an osteoclast.

24. The mixture of cells of claim 1, wherein the non-adipose-derived cell capable of forming a blood cell is selected from the group consisting of a hematopoietic stem cell, megakaryocyte cell and an osteoclast.

25. A mixture of cells comprising an isolated extramedullary adipose tissue-derived stromal cell and a non-adipose derived cell capable of forming a blood cell.

26. A mixture of cells comprising an isolated human extramedullary adipose tissue-derived stromal cell and a non-adipose derived cell capable of forming a blood cell.

27. A mixture of cells comprising an isolated adult human extramedullary adipose tissue-derived stromal cell and a non-adipose derived cell capable of forming a blood cell.

28. A mixture of cells comprising an isolated adult human extramedullary adipose tissue-derived stromal cell and a non-adipose derived human cell capable of forming a blood cell.