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Process for production of mammalian cell lines

  • US 6,555,377 B1
  • Filed: 04/27/2000
  • Issued: 04/29/2003
  • Est. Priority Date: 04/27/1999
  • Status: Expired due to Fees
First Claim
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1. A process for generating a mammalian cell line from primary mammalian cells, comprising:

  • a) pre-treating a culture of primary mammalian cells or a suspension thereof with at least one glucocorticoid, b) if said culture of primary mammalian cells is not in the form of a suspension, obtaining a suspension of the pre-treated culture of step a), c) transferring into the pre-treated cells of the suspension at least one nucleic acid vector which is not of retroviral origin and which is competent to immortalize said pre-treated cells, and d) culturing the transferred cells of step c).

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