Test piece and quantitative method and apparatus for an organism-oriented substance

• US 6,555,383 B2
• Filed: 04/05/2001
• Issued: 04/29/2003
• Est. Priority Date: 12/15/1998
• Status: Expired due to Fees

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Akimoto, Taizo
• Primary Examiner(s): Whisenant, Ethan
• Assistant Examiner(s): LU, FRANK WEI MIN
• Application Number:
  US09/826,349
• Publication Number:
  US 20010018185A1
• Time in Patent Office: 754 Days
• Field of Search: 435/6, 435/91.1, 435/91.2, 435/183, 436/561, 436/94, 536/23.1, 536/24.3, 536/24.33, 536/25.32, 536/25.3
• US Class Current: 436/94
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2563/107 fluorescence
• CPC Class Codes: C12Q 2565/102 Multiple non-interacting labels
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

A plurality of cDNAs each having a known different base sequence are labeled with a fluorescent dye (FITC), whereby F-cDNA is prepared. The F-cDNA is disposed at a plurality of predetermined positions on the slide glass of a DNA micro array chip. Cy5-cDNA is prepared by synthesizing cDNA from poly(A)-mRNA in the presence of a fluorescent dye (Cy5). The Cy5-cDNA is placed on the DNA micro array chip and hybridized to the F-cDNA. After hybridization, the DNA micro array chip is read by a quantitative apparatus incorporated with an analyzer in which information about the base composition ratio and base length of cDNA has been registered.

Claims

1. A measuring method which measures a value detected from a second label corrected for a value detected from a first label comprising the steps of:

detecting a level of a first labeling signal emitted by a first labeling substance, which labels a plurality of known different specific binding substances respectively disposed at a plurality of predetermined positions on a carrier of a test piece, for each of said plurality of predetermined positions;

binding an organism originated substance labeled with a second labeling substance differing from said first labeling substance, to said specific binding substances and detecting a level of a second labeling signal emitted from said second labeling substance for each of said plurality of predetermined positions; and

measuring a quantity of said organism-originated substance bound to said specific binding substance, based on the detected level of said second labeling signal, corrected for the detected level of said first labeling signal.

2. The measuring method as set forth in claim 1, wherein said specific binding substances are cDNA polynucleotides.

3. The measuring method as set forth in claim 1, wherein said method utilizes a correction value calculated from information about 1) the length of the molecule and 2) the relative frequency of that nucleotide within each molecule for each specific binding substance.

4. The measuring method as set forth in claim 2, wherein said method utilizes a correction value calculated from information about 1) the length of the molecule and 2) the relative frequency of that nucleotide within each molecule for each cDNA polynucleotide.

5. The measuring method as set forth in claim 1, wherein said first labeling substance for said specific binding substances is a fluorescent dye.

6. The measuring method as set forth in claim 2, wherein said first labeling substance for said specific binding substances is a fluorescent dye.

7. The measuring method as set forth in claim 3, wherein said first labeling substance for said specific binding substances is a fluorescent dye.

8. The measuring method as set forth in claim 4, wherein said first labeling substance for said specific binding substances is a fluorescent dye.

9. The measuring method as set forth in claim 1, wherein said first labeling substance for said specific binding substances is a radioactive isotope.

10. The measuring method as set forth in claim 2, wherein said first labeling substance for said specific binding substances is a radioactive isotope.

11. The measuring method as set forth in claim 3, wherein said first labeling substance for said specific binding substances is a radioactive isotope.

12. The measuring method as set forth in claim 4, wherein said first labeling substance for said specific binding substances is a radioactive isotope.