Method for determining analyte concentration using periodic temperature modulation and phase detection

• US 6,556,850 B1
• Filed: 09/15/2000
• Issued: 04/29/2003
• Est. Priority Date: 03/12/1997
• Status: Expired due to Fees

• Current Assignee: OptiScan Biomedical Corporation
• Original Assignee: OptiScan Biomedical Corporation
• Inventors: Kramer, Charles E., Braig, James R., Shulenberger, Arthur M., Goldberger, Daniel S., King, Richard A., Trebino, Rick, Zheng, Peng, Sterling, Bernhard B., Barnes, Casper W.
• Primary Examiner(s): WINAKUR, ERIC FRANK
• Application Number:
  US09/663,071
• Time in Patent Office: 956 Days
• Field of Search: 600/309, 600/310, 600/316, 600/322, 600/473, 600/474, 250/341.6, 250/341.1, 250/341.5, 250/339.07, 250/339.09, 250/339.03, 250/339.04, 250/339.05, 250/339.12
• US Class Current: 600/310
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: G01N 21/35 using infrared light G01N21/39 takes precedence

Abstract

A method of determining the analyte concentration of a test sample is described. A temperature gradient is introduced in the test sample and infrared radiation detectors measure radiation at selected analyte absorbance peak and reference wavelengths. Reference and analytical signals are detected. In the presence of the selected analyte, parameter differences between reference and analytical signals are detectable. These parameter differences, having a relationship to analyte concentration, are measured, correlated, and processed to determine analyte concentration in the test sample. Accuracy is enhanced by inducing a periodically modulated temperature gradient in the test sample. The analytical and reference signals may be measured continuously and the parameter difference integrated over the measurement period to determine analyte concentration.

Claims

1. A method of determining the absolute or relative concentration of a chemical analyte in a medium, said method comprising the steps of:

a) inducing at least one thermal gradient into said medium;

b) responsive to said inducing step, measuring the radiation emitted from said medium at a predetermined wavelength related to said analyte, resulting in an analytical signal;

c) Further responsive to said inducing step, measuring the radiation emitted from said medium at least one reference wavelength, resulting in at least one reference signal;

d) comparing the analytical signal with at least one of said at least one reference signals;

e) determining from said comparison, a parameter between said analytical signal and said at least one reference signal; and

f) deducing from said parameter the concentration of said analyte in said medium.

2. A method as in claim 1 wherein said step of inducing at least one thermal gradient into said medium includes inducing a periodically modulated temperature gradient.