Method and device for rapid color detection
First Claim
1. In a method for rapidly detecting light from a channel of capillary dimensions, where a plurality of individual components of a mixture comprising different fluorophores emitting different wavelengths move along said channel at spaced apart intervals for an exposure time of less than about 500 msec, where the light appears as a band, the improvement which comprises:
- dividing the light from said bands into different wavelength ranges, separately detecting each wavelength range with different CCD cameras, where the light illuminates a spot which included from 2 to 100 pixels in a row and binning the signal received along the rows of pixels to provide a total for said band; and
transmitting the signal to a data processor for detecting said band.
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Accused Products
Abstract
Methods and apparatus are provided for miniaturized DNA sequencing using four-color detection. The method employs a multichannel chip where the channels are simultaneously irradiated to excite fluorophores in the channels. The emitted light is divided into four different wavelength beams and read by CCDs.
The results are multiplexed for rapid sequencing of a large number of samples, with high fidelity of the sequences.
219 Citations
17 Claims
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1. In a method for rapidly detecting light from a channel of capillary dimensions, where a plurality of individual components of a mixture comprising different fluorophores emitting different wavelengths move along said channel at spaced apart intervals for an exposure time of less than about 500 msec, where the light appears as a band, the improvement which comprises:
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dividing the light from said bands into different wavelength ranges, separately detecting each wavelength range with different CCD cameras, where the light illuminates a spot which included from 2 to 100 pixels in a row and binning the signal received along the rows of pixels to provide a total for said band; and
transmitting the signal to a data processor for detecting said band. - View Dependent Claims (2, 3, 4, 5, 6, 15)
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7. A method for performing DNA sequencing detecting four different fluorophores emitting at four different wavelengths, said method comprising:
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moving electrophoretically a mixture of fluorophore-labeled oligonucleotides at a rate to provide an exposure time of less than about 500 msec to detection and at a spacing between oligonucleotides in the range of about 50 to 200 μ
m;
irradiating said fluorophore-labeled at a detection site with excitation light to produce bands of emitted light;
dividing the light from said bands into four different wavelength ranges; and
separately detecting the light from each of said bands with different CCD cameras, where the light illuminates a spot which includes from 2 to 100 pixels in a row and binning the signal received along the rows of pixels to provide at total signal for said band. - View Dependent Claims (8, 9, 10, 11, 12)
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13. A method for performing DNA sequencing detecting four different fluorophores emitting at four different wavelengths, said method comprising:
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moving electrophoretically, in a channel having a cross-section in the range of from about 50 to 20,000 μ
2, a mixture of fluorophore-labeled oligonucleotides at a rate to provide and exposure time of less that about 500 msec to detection and at a spacing between oligonucleotides in the range of about 50 to 200 μ
m;
irradiating said fluorophore-labeled at a detection site with an argon laser to produce bands of emitted light;
dividing the light from said bands into four different wavelength ranges; and
separately detecting the light from each of said bands with different CCD cameras, where the light illuminates a spot which includes from 2 to 100 pixels in a row and binning the signal received along the rows of pixels to provide a total signal for said band. - View Dependent Claims (14)
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16. A method for rapidly detecting light from a channel of capillary dimensions, where a plurality of individual components of a mixture comprising different fluorophores emitting different wavelengths move along said channel at spaced apart intervals, the method comprising:
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detecting the light from said channel with more than one CCD cameras where each CCD camera detects light in a certain range and where the light illuminates a spot which includes from 2 to 100 pixels in a row and binning the signal received along the rows of pixels to provide a total signal; and
transmitting the signal to a data processor. - View Dependent Claims (17)
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Specification