pH sensor for indicating the pH of a sample

• US 6,562,297 B1
• Filed: 08/12/1999
• Issued: 05/13/2003
• Est. Priority Date: 08/12/1999
• Status: Expired due to Term

• Current Assignee: Common Sense Ltd.
• Original Assignee: Common Sense Ltd.
• Inventors: Bonstein, Lilach, Kritzman, Amnon
• Primary Examiner(s): SNAY, JEFFREY R
• Application Number:
  US09/372,571
• Time in Patent Office: 1,370 Days
• Field of Search: 422/55, 422/56, 436/163, 436/63, 604/318
• US Class Current: 422/408
• CPC Class Codes: G01N 21/80 Indicating pH value
• CPC Class Codes: G01N 31/221 for investigating pH value

Abstract

A pH sensor for the visual or optical indication of the pH of a sample. The pH sensor includes a hydrophilic, optionally intrinsically charged or neutral, synthetic membrane and at least one pH indicator dye immobilized thereto, so as to prevent appreciated bleeding of the at least one indicator dye from the synthetic membrane upon immersion in an aqueous liquid.

Claims

1. A vaginal secretion monitoring article for providing an indication of a person'"'"'s health, comprising:

a body that includes an absorbent material for absorbing vaginal secretions; and

a pH sensor associated with the body for determining pH of the vaginal secretions, the sensor including a positively charged hydrophilic nylon membrane and at least one indicator dye immobilized thereto so as to prevent appreciated bleeding of the dye from the membrane upon immersion in an aqueous liquid, with the sensor being positioned with respect to the body so as to be wetted by the secretions absorbed by the absorbent material;

wherein the indicator dye changes color to correspond to the pH of the vaginal secretions and the positively charged hydrophilic nylon membrane prevents the color change from fading after drying of the membrane, thereby providing a reliable indication of the health of the person.

2. The article of claim 1 wherein the at least one indicator dye is sensitive to a subtle change in pH of less than N pH units, wherein N is selected from the group consisting of 0.5, 0.4, 0.3, 0.2, 0.1 and 0.05 pH units.

3. The article of claim 1 wherein the dye produces a color change at a pH of between 4 and 5.

4. The article of claim 1 wherein the color of the indicator dye is enhanced after drying of the membrane.

5. The article of claim 1 wherein the pH sensor is externally attached to the body, wherein the body includes a transparent, water-impermeable layer.

6. The article of claim 1 wherein the pH sensor is imbedded between layers of the absorbent material.

7. The article of claim 1 in the form of a hygienic pad or a tampon.

8. The article of claim 1 wherein at least two pH indicator dyes are immobilized onto the membrane.

9. The article of claim 8 wherein the dyes are present as a dye mixture which more accurately measures the pH of the secretions.

10. A vaginal secretion monitoring article for providing an indication of a person'"'"'s health, comprising:

a body that includes an absorbent material for absorbing vaginal secretions; and

a pH sensor associated with the body for determining pH of the vaginal secretions, the sensor including a positively charged hydrophilic nylon membrane and a mixture of at least two indicator dyes immobilized thereto so as to prevent appreciated bleeding of the dye from the membrane upon immersion in an aqueous liquid, with the sensor being positioned with respect to the body so as to be wetted by the secretions absorbed by the absorbent material;

wherein the indicator dye mixture is sensitive to a subtle change in pH of 0.1 pH units or less and changes color to correspond to the pH of the vaginal secretions and the positively charged hydrophilic nylon membrane prevents the color change from fading after drying of the membrane, thereby providing a reliable indication of the health of the person.

11. A method of producing a vaginal secretion monitoring article for providing an indication of a person'"'"'s health, which comprises:

preparing a pH sensor includes a positively charged hydrophilic nylon membrane and at least one indicator dye for determining pH of the vaginal secretions,

immobilizing the dye onto the membrane so as to prevent appreciated bleeding of the dye from the membrane upon immersion in an aqueous liquid; and

associating the sensor with a body that includes an absorbent material for absorbing vaginal secretions, with the sensor being positioned with respect to the body so as to be wetted by the secretions absorbed by the absorbent material;

wherein the indicator dye changes color to correspond to the pH of the vaginal secretions and the positively charged hydrophilic nylon membrane prevents the color change from fading after drying of the membrane, thereby providing a reliable indication of the health of the person.

12. The method of claim 11 which further comprises selecting the at least one indicator dye to be one that is sensitive to a subtle change in pH of less than N pH units, wherein N is selected from the group consisting of 0.5, 0.4, 0.3, 0.2, 0.1 and 0.05 pH units.

13. The method of claim 11 wherein the dye produces a color change at a pH of between 4 and 5.

14. The method of claim 11 wherein the color of the indicator dye is enhanced after drying of the membrane.

15. The method of claim 11 which further comprises externally attaching the pH sensor to the body and providing the body with a transparent, water-impermeable layer.

16. The method of claim 11 which further comprises providing layers of the absorbent material and imbedding the pH sensor between the layers of absorbent material.

17. The method of claim 11 wherein the sensor and body are provided in the form of a hygienic pad or a tampon.

18. The method of claim 11 wherein at least two pH indicator dyes are immobilized onto the membrane.

19. The method of claim 18 wherein the dyes are provided in a dye mixture that is sensitive to a subtle change in pH.

20. The method of claim 19 wherein the dye mixture is provided by:

providing a plurality of buffered solutions of predetermined pH values;

introducing a single, known pH indicator dye into each solution so that the solutions include different pH indicator dyes and optionally different concentrations of the specific pH indicator dyes;

combining at least two solutions that contain different pH indicator dyes to obtain a color;

optically monitoring the color of the combined solution; and

repeating the combining and optically monitoring steps until the resultant solution includes a dye mixture that is sensitive to the subtle change in pH of the vaginal secretions.

21. The method of claim 20 wherein the subtle change in pH is less than N pH units, wherein N is selected from the group consisting of 0.5, 0.4, 0.3, 0.2, 0.1 and 0.05 pH units.