Method of sequencing by oligomer hybridization
First Claim
Patent Images
1. A method of sequencing a DNA fragment by oligomer hybridization, comprising:
- (a) providing a hybridization matrix comprising single-stranded oligomers composed of DNA and RNA nucleotides, wherein all single-stranded oligomers are adjusted to bring said oligomers to a uniform bond strength that is sequence-independent;
(b) exposing a DNA fragment of interest to said hybridization matrix;
(c) determining the positions on said hybridization matrix to which said DNA fragment hybridizes; and
(d) deducing the sequence of said DNA fragment.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates to a method of sequencing DNA by oligomer hybridization, DNA/RNA hybrid oligomers applied onto a support and optionally DNA oligomers and/or RNA oligomers being used as hybridization matrix.
36 Citations
7 Claims
-
1. A method of sequencing a DNA fragment by oligomer hybridization, comprising:
-
(a) providing a hybridization matrix comprising single-stranded oligomers composed of DNA and RNA nucleotides, wherein all single-stranded oligomers are adjusted to bring said oligomers to a uniform bond strength that is sequence-independent;
(b) exposing a DNA fragment of interest to said hybridization matrix;
(c) determining the positions on said hybridization matrix to which said DNA fragment hybridizes; and
(d) deducing the sequence of said DNA fragment. - View Dependent Claims (2, 3, 4, 5, 6, 7)
(a) synthesizing said oligomers of said support individually; and
(b) applying said oligomers on the support surface by means of a linker.
-
-
7. The method of claim 1 or 2, wherein said oligomers shift at least four times from DNA to RNA and from RNA to DNA.
Specification