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Method for quantitative determination of glycated hemoglobin

  • US 6,562,581 B2
  • Filed: 08/31/2001
  • Issued: 05/13/2003
  • Est. Priority Date: 08/31/2001
  • Status: Expired due to Fees
First Claim
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1. A method for quantitative determination of HbA1c without using antibodies comprising:

  • a. incubating a diluted and lysed blood sample comprising a glycated hemoglobin (gHb) with a boronic acid derivative immobilized on a solid support to form a gHb-boronic acid conjugate;

    b. separating the gHb-boronic conjugate from the sample;

    c. adding a dye developing solution that is catalyzed by a peroxidase to form a dye and incubating the gHb-boronic conjugate with the dye developing solution to form a dye; and

    d. measuring the dye concentration, which is directly proportional to the amount of bound glycated hemoglobin.

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