Method for quantitative determination of glycated hemoglobin
First Claim
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1. A method for quantitative determination of HbA1c without using antibodies comprising:
- a. incubating a diluted and lysed blood sample comprising a glycated hemoglobin (gHb) with a boronic acid derivative immobilized on a solid support to form a gHb-boronic acid conjugate;
b. separating the gHb-boronic conjugate from the sample;
c. adding a dye developing solution that is catalyzed by a peroxidase to form a dye and incubating the gHb-boronic conjugate with the dye developing solution to form a dye; and
d. measuring the dye concentration, which is directly proportional to the amount of bound glycated hemoglobin.
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Abstract
A method and a system for separating glycated hemoglobin from non-glycated hemoglobin, and a method for one-read quantitative determination of the glycated hemoglobin and % HbA1c that does not require an additional measurement of total hemoglobin.
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Citations
21 Claims
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1. A method for quantitative determination of HbA1c without using antibodies comprising:
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a. incubating a diluted and lysed blood sample comprising a glycated hemoglobin (gHb) with a boronic acid derivative immobilized on a solid support to form a gHb-boronic acid conjugate;
b. separating the gHb-boronic conjugate from the sample;
c. adding a dye developing solution that is catalyzed by a peroxidase to form a dye and incubating the gHb-boronic conjugate with the dye developing solution to form a dye; and
d. measuring the dye concentration, which is directly proportional to the amount of bound glycated hemoglobin. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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- 14. In a method for quantitative determination of HbA1c without using antibodies, the improvement comprising formation of a detectable gHb-boronic acid conjugate.
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