Probe for localized tissue spectroscopy

• US 6,564,088 B1
• Filed: 01/22/2001
• Issued: 05/13/2003
• Est. Priority Date: 01/21/2000
• Status: Expired due to Fees

• Current Assignee: University Of Massachusetts
• Original Assignee: University Of Massachusetts
• Inventors: Khan, Tania, Soller, Babs R.
• Primary Examiner(s): Hirsch, Paul J.
• Application Number:
  US09/766,990
• Time in Patent Office: 841 Days
• Field of Search: 600/478, 600/477
• US Class Current: 600/478
• CPC Class Codes: A61B 2562/0242 for varying or adjusting the optical path length in the tissue
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: A61B 5/0086 using infrared radiation
• CPC Class Codes: A61B 5/02007 Evaluating blood vessel condition, e.g. elasticity, compliance
• CPC Class Codes: A61B 5/14539 for measuring pH
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence
• CPC Class Codes: A61B 5/6848 Needles
• CPC Class Codes: G01N 21/49 within a body or fluid
• CPC Class Codes: Y10S 977/869 Optical microscope

Abstract

An optical probe structure for illuminating and collecting returned light from a region of tissue. The optical probe is configured with illumination and collection windows to controllably collect light from the region of tissue in vivo. The probe is designed to have a defined spacing between the fiber or fibers connected to the light source, and the fiber or fibers connected to the optical detector, so that light reaching the detector has passed through a localized region of tissue of a small and well-defined size or dimension. The probe can assay or detect tissue cell distribution, a tissue constituent such as hydrogen ions (pH), lactate, or an endogenous tissue component such as hemoglobin, myoglobin, or lipids in plaque. Measurements may indicate conditions such as metabolic state, stress, shock or ischemia in different tissues.

Claims

1. A side-sensing fiber optic probe for interrogating tissue, comprising:

a probe body including a housing configured for holding fiber optics in a side-to-side arrangement,

a first fiber optic enclosed within the housing for delivering light to a region of tissue, and

a second fiber optic enclosed within the housing and held apart by a predetermined spacing from the first fiber optic for receiving light from the region of tissue, the predetermined spacing being based on Monte Carlo simulations to optimize a collected signal for a desired tissue sample,

wherein the housing includes a tissue illumination window for exposing a portion of the first fiber optic, and a detection window for exposing a portion of the second fiber optic, the windows being positioned on a side of the housing so as to allow light to pass through the illumination window from the first fiber optic into the region of tissue and reflect back into the detection window to the second fiber optic when the probe is placed against the tissue.

2. The fiber optic probe of claim 1, wherein the spacing is set to achieve a desired sensitivity to scattering and absorbance.

3. The fiber optic probe of claim 1, wherein the fiber optics are held within grooves in the housing, and the housing contains different spaced grooves to allow selective placement of the first and second fiber optics at different separations to optimize a collected light signal for a given material or tissue.

4. The fiber optic probe of claim 1, wherein the spacing is set within a probe volume corresponding to size of a tissue feature or region of interest.

5. The fiber optic probe of claim 1, configured for penetration of tissue.

6. The fiber optic probe of claim 1, wherein the spacing is set to probe a tissue volume under several cubic millimeters.

7. The fiber optic probe of claim 6, wherein the spacing is set to probe a tissue volume under about one cubic millimeter.

8. The fiber optic probe of claim 7, wherein the spacing is set to probe a tissue volume under about 0.25 cubic millimeters.

9. The fiber optic probe of claim 6, wherein the probe is configured to spectrographically identify and/or measure concentration of one or more materials selected from among a dye, a treatment compound, and a tissue constituent.

10. The fiber optic probe of claim 6, wherein the probe is configured to spectrographically image a region of tissue.

11. The fiber optic probe of claim 1, wherein the windows are symmetrically positioned with respect to a longitudinal axis of the housing.

12. The fiber optic probe of claim 1, configured for direct insertion into a blood vessel to interrogate an internal surface of the vessel wall.

13. A method of analyzing a target tissue by detection of a spectrographic signal of interest, such method comprising the steps of:

placing a probe having a source and a detector in contact with the target tissue, wherein the probe includes a housing for holding apart the source and detector therein, the housing further including an illumination window for exposing a portion of the source and a detection window for exposing a portion of the detector, the windows being positioned on a side of the housing;

setting a spacing between the source and detector for selectively interrogating a limited volume of the target tissue;

illuminating the target tissue with light emitted from the source through the illumination window; and

detecting light through the detection window with the detector, whereby the detected light provides information about the limited volume of target tissue,

wherein the step of setting the spacing includes selecting the spacing based on a non-diffusion modeled interaction to probe a volume localized so as to preferentially detect the signal of interest.

14. The method of claim 13, wherein the probe has plural sets of sources and detectors, each set being positioned to image one of multiple localized volumes in the target tissue for forming a spectrographic image.

15. The method of claim 13, wherein the spectrographic image forms a map of concentration, state or tissue condition.

16. The method of claim 13, wherein the source and detector comprise optical fibers.

17. The method of claim 13, wherein the localized volume corresponds to absorbance depth of a target material.

18. A spectrographic probe for interrogating tissue to detect a signal of interest for spectrographic characterization, the probe comprising:

a probe body including a housing configured for holding an illumination source and a light detector mounted therein, the housing further having an illumination window for exposing a portion of the illumination source and a detection window for exposing a portion of the detector, the windows being positioned on a side of the housing,

the illumination source being positioned by the probe body for delivering light through the illumination window to a region of tissue,

the detector being positioned by the probe body a predetermined spacing from the source for receiving light through the detection window from the region of tissue,

wherein the predetermined spacing is a small spacing effective to limit light received by the detector to light from a tissue depth that preferentially contains the signal of interest, thereby enhancing accuracy of the spectrographic determination.

19. The spectrographic probe of claim 18, wherein the predetermined spacing is set based on Monte Carlo modeling of tissue scattering and an absorbance.

20. The spectrographic probe of claim 18, wherein the source and the detector are optical fibers.

21. The spectrographic probe of claim 18, configured to penetrate tissue.

22. The spectrographic probe of claim 18, configured to contact a tissue surface.

23. The spectrographic probe of claim 22, configured to contact skin and illuminate a volume of target tissue lying below the skin.

24. The spectrographic probe of claim 18, configured for direct insertion into a blood vessel to interrogate an internal surface of the vessel wall.