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Methods of labelling polynucleotides with dibenzorhodamine dyes

  • US 6,566,071 B2
  • Filed: 10/02/2001
  • Issued: 05/20/2003
  • Est. Priority Date: 11/25/1997
  • Status: Expired due to Term
First Claim
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1. A method of forming a dye-labeled polynucleotide comprising:

  • annealing an oligonucleotide primer to a template DNA; and

    extending the primer by polymerase-mediated incorporation of one or more nucleotide 5′

    -triphosphates;

    wherein the primer or at least one of the one or more nucleotide 5′

    -triphosphates comprises a dibenzorhodamine dye comprising the structure;

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    including nitrogen- and aryl-substituted forms thereof;

    whereby a dye-labeled polynucleotide is formed.

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