Cassette for gel electrophoresis having solid buffer reservoirs

• US 6,569,306 B1
• Filed: 04/10/2000
• Issued: 05/27/2003
• Est. Priority Date: 04/10/2000
• Status: Expired due to Fees

• Current Assignee: Hoefer, Inc. (Harvard Bioscience, Inc.)
• Original Assignee: Amersham Pharmacia Biotech Inc. (General Electric Company)
• Inventors: Hennessy, Lori, Islam, Mohammed Rezaul, Read, Doug
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/545,621
• Time in Patent Office: 1,142 Days
• Field of Search: 204/466, 204/456, 204/606, 204/616
• US Class Current: 204/456
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44743 Introducing samples

Abstract

A cassette and electrophoretic gel assembly includes a non-conductive cassette, two solid buffer reservoirs, and an agarose gel. The assembly is disposable, and the sample wells on the gel are in standard microtiter plate format. The configuration is such that the gel is continuously in electrical contact with the electrodes in operation despite the production/migration of water and other exudates.

Claims

1. An electrophoresis device comprising

(a) (i) a non-conductive cassette having a solid buffer reservoir at each of two opposing ends of the cassette, each buffer reservoir having a top surface and a bottom surface, and (ii) a gel having a top surface and a bottom surface, the bottom surface of the gel being in contact with the top surface of the buffer reservoirs;

and

(b) an electrode support supporting two electrodes,

wherein the cassette is adapted to position said gel and buffer reservoirs such that the electrodes of the electrode support will only contact the bottom surface of the buffer reservoirs to permit an electrophoretic procedure to be run, and wherein said gel contains a plurality of wells adapted to contain a plurality of samples.

2. The assembly of claim 1 wherein said gel is an agarose gel.

3. The assembly of claim 1 wherein said gel contains at least 96 wells arranged in a rectangular geometry having rows containing 8 wells and columns containing at least 12 wells.

4. The assembly of claim 3 wherein the columns contain 13 or 14 wells.

5. The assembly of claim 3 wherein the spacing between individual wells within each row and column corresponds to the spacing on a standard microtiter plate.

6. The assembly of claim 1 wherein the cassette comprises a contiguous arrangement of 4 laterally extending outer sides arranged to form a frame.

7. The assembly of claim 6 wherein said sides are sized and arranged so that the frame has a substantially rectangular geometry.

8. The assembly of claim 7 which further includes a gel support platform which extends between one pair of opposing sides.

9. The assembly of claim 8 wherein said platform extends between the pair of sides which have the shorter size.

10. The assembly of claim 9 wherein said platform is recessed below the top surface of said cassette.

11. The assembly of claim 1 wherein said solid buffer reservoirs contain at least 1 ml of buffer.

12. The assembly of claim 11 wherein said solid buffer reservoirs contain different amounts of buffer.

13. The assembly of claim 1 wherein said cassette is comprised of a non-conductive material which is capable of transmitting ultraviolet light.

14. A method of electrophoretically analyzing a plurality of samples with the electrophoresis device of claim 1 comprising the steps of loading the plurality of wells of the cassette with the samples to be analyzed, engaging the cassette with the electrode support such that the electrodes contact the bottom surface of the buffer reservoirs, running the electrophoresis for the desired amount of time, and viewing the results.