Process for mycelial culture using grain

• US 6,569,475 B2
• Filed: 10/05/2001
• Issued: 05/27/2003
• Est. Priority Date: 10/06/2000
• Status: Expired due to Fees

• Current Assignee: CA Biotach Company Limited, Jae-Mahn Song
• Original Assignee: C.A. Biotech Co., Ltd., Jae-Mahn Song
• Inventors: Song, Jae-Mahn, Han, Se-Youn, Na, Yun-Sun
• Primary Examiner(s): Hendricks, Keith
• Application Number:
  US09/971,004
• Publication Number:
  US 20020041917A1
• Time in Patent Office: 599 Days
• Field of Search: 426/44, 426/53, 426/640, 426/52, 426/655, 426/656, 426/618, 435/254.1, 435/256.8, 424/489, 424/93.5, 424/195.1, 424/520, 424/114
• US Class Current: 426/44
• CPC Class Codes: A21D 2/267 Microbial proteins
• CPC Class Codes: A23L 11/50 Fermented pulses or legumes; Fermentation of pulses or legumes based on the addition of microorganisms removing undesirable substances A23L11/30; soy sauce A23L27/50
• CPC Class Codes: A23L 31/00 Edible extracts or preparations of fungi; Preparation or treatment thereof
• CPC Class Codes: A23L 5/00 Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
• CPC Class Codes: A23L 7/104 Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms

Abstract

Disclosed are a method for culturing mushroom mycelia using grains, a culture product, and use of the culture product. Edible or medicinal mushroom mycelia are inoculated and cultured in solid media made of grains. Induction of the cultured mushroom mycelia to undergo autolysis produces autolysates rich in antitumorigenic and other medicinally useful materials. The squeezing of the autolysates produces a liquid filtrate, leaving a paste. The filtrate is concentrated for use in foods or medicines. The paste is processed into a nutrient-rich gruel or other foods.

Claims

1. A method for preparing products from cultured mushroom mycelia, comprising the steps of:

preparing a solid medium by boiling or soaking a grain in 2 or 3 equivalents of water, placing the grain in a culture vessel, autoclaving and cooling the grain;

inoculating a mushroom mycelium in an incubator at 20 to 30°

C. for 15 to 30 days to root the mushroom mycelium into the solid medium;

inducing autolysis of the mushroom mycelium grown into the grain substrate medium by treating the culture vessel at 45 to 55°

C. for 24 to 48 hours;

squeezing the autolysate to produce a liquid and concentrating the liquid; and

drying the paste to a powder, wherein the paste is the solid extract from which the liquid is removed.

2. The method as set forth in claim 1, wherein the grain is selected from the group consisting of rice, barley, wheat, millet, sorghum, Job'"'"'s-tears, beans and corn.

3. The method as set forth in claim 1, wherein the mushroom mycelium is selected from the group consisting of Coriolus versicolor, Ganoderma lucidum, Schizophyilum commune, Pieurotus ostreatus, Pleurotus sajor-caju, Lentinus edodes (Berk), Flammulina velutipes, Auricularia spp., and Pholiota nameko.

4. A method for preparing a gruel from a mixture of a grain and autolysed mushroom mycelium, comprising the steps of:

preparing a solid medium by boiling or soaking a grain in 2 to 3 weights of water, placing the grain in a culture vessel, autoclaving and cooling the grain;

inoculating a mushroom mycelium into the solid medium and culturing the mushroom mycelium in an incubator at 20 to 30°

C. for 15 to 30 days to root the mushroom mycelium into the solid medium;

mixing the mushroom culture, the grain, and water in proportions of 4;

4;

1;

incubating the mixture at 60°

C. for 16 hours to induce the autolysis of the mushroom mycelium and the hydrolysis of the grain, simultaneously; and

heating the autolysates and hydrolysates at 80°

C. for 30 mm to give a gruel.

6. The method as set forth in claim 4, wherein the grain is selected from the group consisting of rice, barley, wheat, millet, sorghum, Job'"'"'s-tears, beans and corn.

7. The method as set forth in claim 4, wherein the mushroom mycelium is selected from the group consisting of Coriolus versicolor, Ganoderma lucidum, Schizophyllum commune, Pleurotus ostreatus, Pleurotus sajor-caju, Lentinus edodes (Berk) Flammulina velutipes, Auricularia spp., and Pholiota nameko.

5. A method for preparing a powder from a dried mixture of a grain and autolysed mushroom mycelium, comprising the steps of:

preparing a solid medium by boiling or soaking a grain in 2 to 3 weights of water, placing the grain in a culture vessel, autoclaving and cooling the grain;

inoculating a mushroom mycelium into the solid medium and culturing the mushroom mycelium in an incubator at 20 to 30°

C. for 15 to 30 days to root the mushroom mycelium into the solid medium;

mixing the mushroom culture, the grain, and water in proportions of 4;

4;

1;

incubating the mixture at 60°

C. for 16 hours to induce the autolysis of the mushroom mycelium and the hydrolysis of the grain, simultaneously;

heating the autolysates and hydrolysates at 80°

C. for 30 mm to give a gruel; and

drying the gruel with hot air to produce a gruel powder.