Pressure cycling reactor

• US 6,569,672 B1
• Filed: 01/21/2000
• Issued: 05/27/2003
• Est. Priority Date: 03/07/1995
• Status: Expired due to Term

• Current Assignee: BBI Bioseq Incorporated
• Original Assignee: BBI Bioseq Incorporated
• Inventors: Green, David J., Dreier, Gustav H., Rudd, Edwin A., Laugharn, James A. Jr.
• Primary Examiner(s): Redding, David A.
• Application Number:
  US09/489,586
• Time in Patent Office: 1,222 Days
• Field of Search: 435/286.6, 435/287.2, 435/288.7, 422/295, 422/309
• US Class Current: 435/286.6
• CPC Class Codes: B01J 19/185 of the pulsating type
• CPC Class Codes: B01J 2219/00162 controlling the pressure
• CPC Class Codes: B01J 2219/002 inside the reactor
• CPC Class Codes: B01J 2219/00211 comparing a sensed parameter with a pre-set value
• CPC Class Codes: B01J 2219/00229 of the reaction system
• CPC Class Codes: B01J 3/00 Processes of utilising sub-atmospheric or super-atmospheric pressure to effect chemical or physical change of matter; Apparatus therefor pressure vessels for containing or storing compressed, liquefied or solidified gases F17C
• CPC Class Codes: C12N 9/00 Enzymes; Proenzymes; Compositions thereof preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113; Processes for preparing, activating, inhibiting, separating or purifying enzymes preparation of malt C12C1/00
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

Methods and apparatus in which pressure provides precise control over the timing and preferably synchronization of chemical reactions, particularly enzymatic reactions.

Claims

1. A pressure cycling reactor for conducting a controlled pressure sensitive reaction comprising,

a reaction vessel for containing a sample,

a vessel pressurizer connected to the reaction vessel, and

a controller connected to the vessel pressurizer for signalling the pressurizer to maintain reaction inactivating pressure P₁ in the reaction vessel and signalling the pressurizer to change pressure in the reaction vessel to a reaction activating pressure P₂ for a pulsed period t₁ and then recycling to a reaction inactivating pressure P₃ in the reaction vessel.

2. The pressure cycling reactor of claim 1 wherein said pressurizer is capable of achieving a change in pressure of over 20,000 psi in less than 250 milliseconds.

3. The pressure cycling reactor of claim 1 further including a fluid source in communication with said reaction vessel.

4. The pressure cycling reactor of claim 1 wherein said vessel pressurizer includes a pressure chamber, said pressure chamber being connected to said reaction vessel such that said pressure chamber communicates with said reaction vessel responsive to said controller.

5. The pressure cycling reactor of claim 4 wherein said pressure chamber comprises a pneumatic pump cylinder.

6. The pressure cycling reactor of claim 4 wherein said pressure chamber has a variable volume, said pressurizer further including a pressure transmitter for connection to a pressure source, said pressure transmitter being connected to a pressure chamber wall to control the position of the wall and thereby control the pressure chamber volume.

7. The pressure cycling reactor of claim 6 wherein said pressure transmitter comprises a pneumatic cylinder.

8. The pressure cycling reactor of claim 6 further including a relief valve for adjusting the pressure in said pressure transmitter thereby creating a pressure pulse in said reaction vessel.

9. The pressure cycling reactor of claim 4 further including a pressure chamber outlet valve positioned between said pressure chamber and said reaction vessel.

10. The pressure cycling reactor of claim 4 further including a fluid source in communication with said reaction vessel, and a pressure chamber inlet valve positioned between said fluid source and said pressure chamber.

11. The pressure cycling reactor of claim 10 wherein said fluid source comprises a fluid reservoir connected to a reservoir pressure source, and a reservoir control valve, whereby fluids are moved from said fluid reservoir to said reaction vessel via said pressure chamber, while pressure in said reaction vessel is controlled.

12. The pressure cycling reactor of claim 11, further comprising a temperature sensor connected to said fluid reservoir and a reservoir vessel heating and/or cooling source, whereby the temperature of the fluid in the reservoir is controlled.

13. The pressure cycling reactor of claim 1 further including a pressure sensor connected to said reaction vessel and to said controller, whereby the controller monitors the pressure in said reaction vessel.

14. The pressure cycling reactor of claim 13 further including a pressure regulator for adjusting the pressure in said pressure chamber, said controller using feedback from said pressure sensor to control said pressure regulator.

15. The pressure cycling reactor of claim 1, wherein said vessel pressurizer is a first vessel pressurizer, said reactor further comprising a second vessel pressurizer connected to the reaction vessel, whereby fluids are removed from said reaction vessel while pressure in said reaction vessel is controlled.

16. The pressure cycling reactor of claim 15 further including a second vessel pressurizer inlet valve located between said reaction vessel and said second vessel pressurizer.

17. The pressure cycling reactor of claim 15 further including a second fluid reservoir and a second vessel pressurizer outlet valve located between said second fluid reservoir and said second vessel pressurizer.

18. The pressure cycling reactor of claim 17 further including a second reservoir control valve connected to said second fluid reservoir.

19. The pressure cycling reactor of claim 15 wherein said controller controls a plurality of valves according to a predetermined set of stored signals which control the valves to add at least one pressurized reagent fluid to the reaction vessel and to remove at least one pressurized reacted fluid from the reaction vessel, while the reaction vessel remains under pressure.

20. The pressure cycling reactor of claim 1, further comprising a temperature sensor connected to said reaction vessel and a reaction vessel heating and/or cooling source, whereby the temperature of the reaction vessel is controlled.

21. The pressure cycling reaction of claim 1, further comprising a detector connected to the reaction vessel in a manner that allows it to detect a characteristic of a component present in a sample in or removed from the reaction vessel.

22. The pressure cycling reactor of claim 21 in which the detector is selected from the group consisting of a radioisotopic detector, an infra-red spectrometer, a mass spectrometer, a gas chromatography-mass spectrometer, a spectrophotometer, a spectrofluorometer, an electrochemical detector, a surface plasmon resonance detector, and a photometer.

23. The pressure cycling reactor of claim 1 wherein said reaction vessel includes a restraint to retain an immobilized reagent within the reaction vessel while permitting removal of fluid from the vessel.

24. The pressure cycling reactor of claim 23 wherein said immobilized reagent comprises an organic compound attached to a non-liquid support.

25. The pressure cycling reactor of claim 23 wherein said restraint comprises a semi-permeable barrier which divides the reaction vessel into two segments.

27. The pressure cycling reactor of claim 1 wherein said vessel pressurizer is a first vessel pressurizer, said reactor further comprising a second vessel pressurizer connected to the reaction vessel, whereby fluids are introduced into said reaction vessel while pressure in said reaction vessel is controlled.

26. A reactor for intermittently inhibiting activity of a sample by controlling temperature and pressure conditions of the sample, comprising:

a reactor body defining a chamber for receiving the sample,

a pressurizer in communication with said chamber mounted for movement between a first position for applying a first predetermined pressure P₁ to said chamber, said first predetermined pressure P₁ being selected to inhibit activity of the sample, and a second position for changing said chamber pressure to a second predetermined pressure P₂ selected to allow activity of the sample,

a switch having a first state and a second state to move said pressurizer between said first position and said second position,

a controller for changing said switch between said first and second states,

means for adjusting temperature in said chamber, and

a port in communication with said chamber for removal of the sample from said chamber.