Dual manifold system and method for fluid transfer

• US 6,569,687 B2
• Filed: 12/15/2000
• Issued: 05/27/2003
• Est. Priority Date: 03/04/1999
• Status: Expired due to Fees

• Current Assignee: UT-Battelle LLC
• Original Assignee: UT-Battelle LLC
• Inventors: Doktycz, Mitchel J., Kress, Reid, Bryan, William Louis
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): GORDON, BRIAN R
• Application Number:
  US09/738,821
• Publication Number:
  US 20010053337A1
• Time in Patent Office: 893 Days
• Field of Search: 422/100, 436/180, 436/174, 738/641.7, 738/633.2, 738/64, 738/640.1, 738/640.2, 738/641.1
• US Class Current: 436/180
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00315 Microtiter plates
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01L 3/0241 Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A dual-manifold assembly is provided for the rapid, parallel transfer of liquid reagents from a microtiter plate to a solid state microelectronic device having biological sensors integrated thereon. The assembly includes aspiration and dispense manifolds connected by a plurality of conduits. In operation, the aspiration manifold is actuated such that the aspiration manifold is seated onto an array of reagent-filled wells of the microtiter plate. The wells are pressurized to force reagent through conduits toward the dispense manifold. A pressure pulse provided by a standard ink-jet printhead ejects nanoliter-to-picoliter droplets of reagent through an array of printhead orifices and onto test sites on the surface of the microelectronic device.

Claims

1. A method for transferring liquid reagent from a first test site to a second test site on a target substrate using a dual-manifold assembly, comprising the steps of:

positioning at least one source reservoir relative to an aspiration manifold, said source reservoir having at least one reagent therein, said aspiration manifold having at least one aspiration conduit;

positioning said target substrate relative to a dispense manifold having at least one channel terminating at an orifice wherein at least one of said dispense manifold channel and said aspiration conduit number at least two;

seating said aspiration conduit onto said source reservoir;

applying pressure to at least a first location to force said source reservoir to flow said reagent via said aspiration conduit to said dispense manifold; and

,

applying pressure to at least a second location, said second location different from said first location, to force said reagent in said dispense manifold channel to eject a desired volume of said reagent to said second test site.

2. The method as recited in claim 1, further comprising the step of checking the alignment of said target substrate prior to said applying pressure to said second location.

3. The method as recited in claim 1, further comprising the step of detecting the passage of a volume of reagent from said orifice to said second test site after applying pressure at said second location.

4. The method as recited in claim 1, wherein said second location is disposed between said aspiration and said dispense manifold, further comprising the step of purging at least a portion of said aspiration conduit with a purging gas or a cleaning solution, said purging gas or said cleaning solution introduced at said second location.

5. The method as recited in claim 1, wherein said applying pressure at said second location step comprises communicating a pressure pulse in the range of about 6.9(10)³ N/m² to about 138(10)³ N/m², for a period of time in the range of about (10)⁻

6 seconds to about (10)<sup>−

2</sup> seconds.

6. The method of claim 1, wherein said dispense manifold channel is spaced apart from said reagent in said second test site during an entire duration of said method.

7. The method of claim 1, further comprising the step of disabling fluid connection between said dispense manifold and said aspiration manifold.

8. The method of claim 1, wherein said at least one aspiration conduit comprises a plurality of aspiration conduits, said applying pressure at said first location step comprising pressurizing at least one of said plurality of aspiration conduits differently as compared to any of the other of said plurality of aspiration conduits, wherein different pressures or times at pressure can be selectively applied to respective ones of said plurality of aspiration conduits.

9. The method of claim 1, wherein said at least one dispense manifold channel comprises a plurality of dispense manifold channels, said applying pressure to said second location step comprises pressurizing at least one of said plurality of dispense manifold channels differently as compared to any of the other of said plurality of dispense manifold channels, wherein different pressures or times at pressure can be selectively applied to respective ones of said plurality of dispense manifold channels.