Claudin-50 protein

• US 6,573,068 B1
• Filed: 09/15/2000
• Issued: 06/03/2003
• Est. Priority Date: 11/13/1997
• Status: Expired due to Fees

• Current Assignee: Serono Genetics Institute S.A. (Merck & Co., Inc.)
• Original Assignee: S. A. Genset
• Inventors: Milne Edwards, Jean-Baptiste Dumas, Bougueleret, Lydie, Duclert, Aymeric
• Primary Examiner(s): Eyler, Yvonne
• Assistant Examiner(s): O HARA, EILEEN B
• Application Number:
  US09/663,600
• Time in Patent Office: 991 Days
• Field of Search: 530/350, 536/23.5, 435/69.1, 435/320.1, 435/325
• US Class Current: 435/69.1
• CPC Class Codes: C07K 14/47 from mammals

Abstract

The sequence of a cDNA encoding Claudin-50 and sequence of Claudin-50 protein are disclosed. The cDNA can be used to express the protein or portions thereof or to obtain antibodies capable of specifically binding to the secreted protein. The cDNA may also be used in diagnostic, forensic, gene therapy, and chromosome mapping procedures. The cDNA may also be used to design expression vectors and secretion vectors.

Claims

1. A purified and isolated Claudin polypeptide comprising an amino acid sequence of positions 1 to 206 of SEQ ID NO:

186.

2. The polypeptide of claim 1, wherein said polypeptide comprises an amino acid sequence of positions −

24 to 206 of SEQ ID NO;

186.

3. A method of producing the polypeptide of claim 2, comprising the steps of:

(i) obtaining a host cell capable of expressing said polypeptide;

(ii) culturing said cell under conditions suitable for the expression of said polypeptide; and

(iii) isolating said polypeptide from said host cell.

4. A method of producing the polypeptide of claim 1 comprising the steps of:

(i) obtaining a host cell capable of expressing said polypeptide;

(ii) culturing said cell under conditions suitable for the expression of said polypeptide; and

(iii) isolating said polypeptide from said host cell.