Methods and apparatus for uniform transcutaneous therapeutic ultrasound
First Claim
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1. A method of enhancing transfection of DNA delivered into a target region of a patient'"'"'s body, comprising:
- delivering the DNA into the target region; and
applying a uniform field of ultrasound energy across a wide area of the target region, the ultrasound energy being of a type and an amount sufficient to enhance transfection of the DNA in the target region, wherein the uniform field comprises a field which varies in intensity by at least one of (1) less than 10 dB in a lateral direction across the width of the field, and (2) less than 10 dB in an axial direction across the depth of the target region.
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Abstract
A wide beam ultrasound delivery system providing a uniform exposure field is used to enhance the uptake of injected substances and/or to enhance the transfection of DNA in the tissues of human subject, or reduce the amount of vascular intimal hyperplasia in human subjects following vascular injury.
231 Citations
31 Claims
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1. A method of enhancing transfection of DNA delivered into a target region of a patient'"'"'s body, comprising:
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delivering the DNA into the target region; and
applying a uniform field of ultrasound energy across a wide area of the target region, the ultrasound energy being of a type and an amount sufficient to enhance transfection of the DNA in the target region, wherein the uniform field comprises a field which varies in intensity by at least one of (1) less than 10 dB in a lateral direction across the width of the field, and (2) less than 10 dB in an axial direction across the depth of the target region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
contacting a window of a housing on a patient'"'"'s skin; and
driving an ultrasound transducer mounted within the housing to deliver the ultrasound energy.
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27. The method of claim 1, wherein the uniform field of ultrasound is applied across the wide area of the target region concurrently with delivering the DNA into the target region.
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28. The method of claim 1, wherein the uniform field of ultrasound is applied across the wide area of the target region before delivering the DNA into the target region.
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29. The method of claim 1, wherein the uniform field of ultrasound is applied across the wide area of the target region immediately after delivering the DNA into the target region.
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30. The method of claim 1, wherein the uniform field of ultrasound is applied across the wide area of the target region 15 to 60 minutes time after delivering the DNA into the target region.
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31. A kit for enhancing transfection of DNA delivered into a target region of a patient'"'"'s body, comprising:
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an ultrasound energy delivery system; and
instructions for use setting forth the method of claim 1.
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Specification