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Methods and compositions for the detection of chromosomal aberrations

  • US 6,576,421 B1
  • Filed: 02/11/2000
  • Issued: 06/10/2003
  • Est. Priority Date: 10/28/1991
  • Status: Expired due to Term
First Claim
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1. A method of detecting in a sample of chromosomes a chromosomal translocation associated with cancer, wherein the translocation results in at least one fusion chromosome, said method comprising:

  • (a) preparing at least a first, a second and a third genomic nucleic acid probe, said first and second probe designed to hybridize to a fusion chromosome on one side of the fusion breakpoint region, but at different locations and said third probe is designed to hybridize to a chromosome on the other side of the breakpoint fusion region, wherein said first, second and third probes are distinctly labeled so that each is distinguishable;

    (b) contacting the labeled probes in pairs wherein the third probe is a member of each pair, to the sample containing the chromosomes under conditions of appropriate stringency to allow specific hybridization of the probes to complementary sequences within the DNA of said chromosomes; and

    (c) detecting the presence of label from the three probes hybridized to the chromosomes; and

    (d) inferring the presence of the translocation from the label pattern.

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