Filtration-detection device and method of use

• US 6,576,460 B1
• Filed: 10/27/2000
• Issued: 06/10/2003
• Est. Priority Date: 10/28/1999
• Status: Expired due to Fees

• Current Assignee: Cornell Research Foundation Incorporated (Cornell University), Innovative Biotechnologies International, Inc. (Rheonix, Inc.)
• Original Assignee: Cornell Research Foundation Incorporated (Cornell University), Innovative Biotechnologies International, Inc. (Rheonix, Inc.)
• Inventors: Baeumner, Antje J., Montagna, Richard A.
• Primary Examiner(s): Le, Long V.
• Assistant Examiner(s): Padmanabhan, Kartic
• Application Number:
  US09/698,564
• Time in Patent Office: 956 Days
• Field of Search: 210/348, 210/500.1, 210/500.21, 210/500.27, 210/500.29, 210/502.1, 210/506, 210/634, 210/638, 210/644, 210/645, 210/649-651, 210/653, 210/655, 210/660, 210/679, 210/690, 210/767, 210/902, 210/908, 422/55, 422/57, 422/58, 422/68.1, 422/76, 422/77, 422/82-1-, 422/82.11, 422/90, 422/91, 422/98, 422/101, 422/119, 422/255, 422/939, 435/287.1, 435/287.2, 435/287.9, 435/288.5, 435/288--28, 435/808, 435/814, 435/817, 435/822, 436/149-151, 436/164, 436/169, 436/172, 436/177, 436/178, 436/518, 436/528, 436/529, 436/530, 436/532, 436/535, 436/536, 436/538, 436/541, 436/805, 436/806, 436/823, 436/824, 204/193, 204/194, 204/400, 204/406, 204/403, 204/407, 204/409-412, 204/415, 204/418, 204/419, 204/422, 204/424, 204/426, 204/435, 204/228.1, 204/229.6, 204/229.4, 204/229.8, 204/230.2, 204/298.03, 204/298.32, 204/660, 204/665, 204/672
• US Class Current: 435/287.1
• CPC Class Codes: G01N 33/5304 Reaction vessels, e.g. agglutination plates for solid-phase systems G01N33/543
• CPC Class Codes: G01N 33/5432 Liposomes or microcapsules
• CPC Class Codes: Y10S 210/902 Materials removed
• CPC Class Codes: Y10S 210/908 Organic
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 435/814 Enzyme separation or purification
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode
• CPC Class Codes: Y10S 435/822 using bacteria or actinomycetales
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property
• CPC Class Codes: Y10S 436/823 Immunogenic carrier or carrier per se
• CPC Class Codes: Y10S 436/824 Immunological separation techniques
• CPC Class Codes: Y10S 977/753 with polymeric or organic binder
• CPC Class Codes: Y10S 977/953 Detector using nanostructure

Abstract

The present invention relates to a filtration-detection device for detecting or quantifying an analyte in a test sample including a filtration device having a first binding material immobilized thereto, wherein the first binding material is capable of binding to a portion of the analyte, and a detection assembly positioned relative to the filtration device to detect or quantify analyte bound to the first binding material. The present invention also relates to methods of using the filtration-detection device.

Claims

1. A filtration-detection device for detecting or quantifying an analyte in a test sample comprising:

a filtration device comprising a porous membrane having a first binding material immobilized thereto, wherein the porous membrane is supported on a support having an opening for passage of the test sample away from the porous membrane, and wherein the first binding material binds to a portion of the analyte; and

a detection assembly movable between a first position out of contact with the filtration device and a second position in electrolytic contact with the filtration device to detect or quantify analyte bound to the first binding material.

2. A device according to claim 1, wherein said detection assembly is in contact with the filtration device.

3. A device according to claim 1, wherein said filtration device comprises a nitrocellulose membrane.

4. A device according to claim 1, wherein said first binding material is selected from the group consisting of an antibody, an antigen, a nucleic acid sequence, an aptamer, and a cell receptor.

5. A device according to claim 1, wherein said analyte is a target nucleic acid molecule and said first binding material is a capture probe selected to hybridize with a portion of said target nucleic acid molecule.

6. A device according to claim 5, wherein said target nucleic acid molecule is found in an organism selected from the group consisting of bacteria, fungi, viruses, protozoa, parasites, animals, and plants.

7. A device according to claim 6, wherein said organism is Cryptosporidium parvum.

8. A device according to claim 1, wherein said detection assembly is an electrochemical detection assembly.

9. A device according to claim 8, wherein said electrochemical detection assembly comprises an electrode array comprising a first conductor having a plurality of fingers and a second conductor having a plurality of fingers, wherein said fingers of said first conductor are interdigitated with said fingers of said second conductor, said first and second conductors are electrically connected to one another via a voltage source and readout device, and said array is positioned to induce redox cycling of an electroactive marker.

10. A device according to claim 9, wherein said device further comprises a reference electrode electrically connected to said electrode array.

11. A device according to claim 9, wherein either or both of said first conductor and said second conductor comprise one or more materials selected from the group consisting of platinum, gold, graphite, and silver.

12. A device according to claim 9, wherein each of said first conductor and said second conductor comprise from 2 to 1000 fingers.

13. A device according to claim 9, wherein said fingers of each of said first conductor and said second conductor are from about 1 μ

m to about 20 μ

m wide and are spaced from about 1 nm to about 10 μ

m apart.

14. A device according to claim 9, wherein each of said first conductor and said second conductor further comprises a contact pad through which the fingers are electrically connected to the voltage source, and wherein said contact pad is at least partially coated with an insulating material.

15. A device according to claim 1, wherein said detection assembly is an optical detection assembly.