Electrochemical affinity assay
First Claim
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1. A method for detecting a second member of a ligand—
- ligand receptor pair in a sample;
the method comprising;
contacting the sample with an electrode having immobilized thereon a first member of the ligand—
ligand receptor pair and with a third member that binds the second member to form a first member-second member-third member complex, wherein the first member is immobilized in a redox polymer and the third member is labeled with a detection marker;
applying a potential to the electrode, wherein the applied potential causes the detection marker-labeled first member-second member-third member complex to generate an electrical signal; and
correlating the generated electrical signal with the presence or amount of the second member in the sample.
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Abstract
An electrochemical affinity assay system for detection of ligand—ligand receptor binding.
116 Citations
34 Claims
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1. A method for detecting a second member of a ligand—
- ligand receptor pair in a sample;
the method comprising;contacting the sample with an electrode having immobilized thereon a first member of the ligand—
ligand receptor pair and with a third member that binds the second member to form a first member-second member-third member complex, wherein the first member is immobilized in a redox polymer and the third member is labeled with a detection marker;
applying a potential to the electrode, wherein the applied potential causes the detection marker-labeled first member-second member-third member complex to generate an electrical signal; and
correlating the generated electrical signal with the presence or amount of the second member in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
- ligand receptor pair in a sample;
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19. A method for detecting a second member of a ligand—
- ligand receptor pair in a sample;
the method comprising;contacting the sample with a sensor wherein the sensor comprises;
an electrode;
a redox polymer film on the electrode;
a substrate generating enzyme on the electrode that generates a detection compound;
a first member of a ligand—
ligand receptor pair that binds the second member,wherein the first member is immobilized in the redox polymer on the electrode;
and with a third member that binds the second member to form a first member-second member-third member complex, the third member labeled with a detection marker that reacts with the detection compound;
applying a potential to the electrode, wherein the applied potential causes the detection marker-labeled first member-second member-third member complex to generate and electrical signal; and
correlating the generated electrical signal with the presence or amount of the second member in the sample. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
- ligand receptor pair in a sample;
Specification