Method for determining analyte concentration using periodic temperature modulation and phase detection

• US 6,577,885 B1
• Filed: 09/15/2000
• Issued: 06/10/2003
• Est. Priority Date: 03/12/1997
• Status: Expired due to Fees

• Current Assignee: OptiScan Biomedical Corporation
• Original Assignee: OptiScan Biomedical Corporation
• Inventors: Kramer, Charles E., Braig, James R., Zheng, Peng, Sterling, Bernhard B., Shulenberger, Arthur M., Goldberger, Daniel S., King, Richard A., Trebino, Rick, Barnes, Casper W.
• Primary Examiner(s): WINAKUR, ERIC FRANK
• Application Number:
  US09/663,114
• Time in Patent Office: 998 Days
• Field of Search: 600/309, 600/310, 600/316, 600/322, 600/473, 600/474, 250/341.6, 250/341.1, 250/341.5, 250/339.07, 250/339.09, 250/339.03, 250/339.04, 250/339.05, 250/339.12
• US Class Current: 600/310
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: G01N 21/35 using infrared light G01N21/39 takes precedence

Abstract

A method of determining the analyte concentration of a test sample is described. A temperature gradient is introduced in the test sample and infrared radiation detectors measure radiation at selected analyte absorbance peak and reference wavelengths. Reference and analytical signals are detected. In the presence of the selected analyte, parameter differences between reference and analytical signals are detectable. These parameter differences, having a relationship to analyte concentration, are measured, correlated, and processed to determine analyte concentration in the test sample. Accuracy is enhanced by inducing a periodically modulated temperature gradient in the test sample. The analytical and reference signals may be measured continuously and the parameter difference integrated over the measurement period to determine analyte concentration.

Claims

1. A method of analyzing a test sample utilizing a radiation detector, comprising the steps of:

a) providing a test sample;

b) inducing a thermal gradient in said test sample;

c) responsive to said inducing step, measuring at least one reference signal emitted by said sample;

d) further responsive to said inducing step, measuring at least one analytical signal emitted by said sample;

e) comparing said reference and analytical signals to determine parameter information; and

f) transmitting said parameter information, as an electrical signal for further processing.

2. A method as in claim 1 wherein said step (e) of comparing said reference and analytical signals to determine parameter information includes comparing said reference and analytical signals to determine phase difference information between said analytical and reference signals.

3. A method as in claim 2 wherein said step of comparing phase difference information includes the step of deducing the analyte concentration of said sample based on said phase difference information.

4. A method as in claim 3 wherein said step of deducing the analyte concentration includes correlating said phase information with known information regarding analyte concentration and phase.

5. A method as in claim 2 wherein said at least one analytical signal is measured at one or more predefined wavelengths.

6. A method as in claim 3 wherein said predefined wavelength intervals are defined by analyte absorbance peaks.

7. A method as in claim 2 wherein said at least one reference signal is measured at one or more predefined wavelength intervals.

8. A method as in claim 7 wherein said predefined wavelength intervals are defined by the absorbance properties of the sample media.

9. A method as in claim 1 wherein said step of inducing a thermal gradient in said test sample includes inducing a periodic thermal gradient in said test sample.