Integrated monolithic microfabricated electrospray and liquid chromatography system and method

• US 6,579,452 B1
• Filed: 11/07/2000
• Issued: 06/17/2003
• Est. Priority Date: 09/17/1998
• Status: Expired due to Term

• Current Assignee: Rheonix, Inc.
• Original Assignee: Advion BioSciences Incorporated, Calient Networks Incorporated
• Inventors: Schultz, Gary A., Davis, Timothy J., Moon, James E., Corso, Thomas N., Galvin, Gregory J., Lowes, Stephen
• Primary Examiner(s): Therkorn, Ernest G.
• Application Number:
  US09/707,653
• Time in Patent Office: 952 Days
• Field of Search: 250/288, 250/288.A, 210/656, 210/748, 210/198.2, 210/243, 204/600, 422/70, 436/161
• US Class Current: 210/198.2
• CPC Class Codes: G01N 2030/6013 interfaces to detectors
• CPC Class Codes: G01N 30/6095 Micromachined or nanomachined, e.g. micro- or nanosize
• CPC Class Codes: G01N 30/7266 by electric field, e.g. electrospray
• CPC Class Codes: H01J 49/0018 Microminiaturised spectrometers, e.g. chip-integrated devices, Micro-Electro-Mechanical Systems [MEMS]
• CPC Class Codes: H01J 49/167 Capillaries and nozzles specially adapted therefor; electrostatic spraying per se B05B5/00
• CPC Class Codes: Y10T 436/24 Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

An electrospray device, a liquid chromatography device and an electrospray-liquid chromatography system are disclosed. The electrospray device comprises a substrate defining a channel between an entrance orifice on an injection surface and an exit orifice on an ejection surface, a nozzle defined by a portion recessed from the ejection surface surrounding the exit orifice, and an electrode for application of an electric potential to the substrate to optimize and generate an electrospray; and, optionally, additional electrode(s) to further modify the electrospray. The liquid chromatography device comprises a separation substrate defining an introduction channel between an entrance orifice and a reservoir and a separation channel between the reservoir and an exit orifice, the separation channel being populated with separation posts perpendicular to the fluid flow; a cover substrate bonded to the separation substrate to enclose the reservoir and the separation channel adjacent the cover substrate; and, optionally, electrode(s) for application of a electric potential to the fluid. The exit orifice of the liquid chromatography device may be homogeneously interfaced with the entrance orifice of the electrospray device to form an integrated single system. An array of multiple systems may be fabricated in a single monolithic chip for rapid sequential fluid processing and generation of electrospray for subsequent analysis, such as by positioning the exit orifices of the electrospray devices near the sampling orifice of a mass spectrometer.

Claims

1. A system comprising:

a microfabricated device defining a liquid chromatography device comprising an entrance for receiving fluid containing an analyte, the device further defining a monolithic electrospray device having an entrance orifice on an injection side and a nozzle on an ejection surface on an opposite planar side from the injection side, a channel extending continuously through the monolithic device in communication with the entrance orifice and the nozzle, and a region surrounding the nozzle recessed from the ejection surface, the monolithic electrospray device being configured to receive the fluid containing analyte from the liquid chromatography device and to generate an electrospray from the fluid; and

a mass spectrometer comprising a sampling orifice, said microfabricated device being positioned to eject the electrospray from the nozzle into the sampling orifice.