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Method for label-free detection of hybridized DNA targets

  • US 6,579,680 B2
  • Filed: 02/28/2001
  • Issued: 06/17/2003
  • Est. Priority Date: 02/28/2000
  • Status: Expired due to Fees
First Claim
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1. A method for detecting or identifying genetic polymorphisms, said method comprising:

  • providing (1) a set of first probes, each (i) having a nucleic acid sequence that is attached to a solid support, and (ii) differing from each other in at least one base location, and (2) a set of second probes, each having a nucleic acid sequence that incorporates at least one artificial mismatch site relative to a first probe, such that said first probe and second probe are bound to each other in a duplex;

    providing each first probe with an emitter element, and each second probe with a quencher element;

    hybridizing an unlabeled sample nucleic acid target to said duplex of first and second probes;

    displacing said second probe from said duplex with said sample nucleic acid target, wherein said emitter element increases in emission intensity with such displacement.

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