Device for automated detection of nucleic acids

• US 6,586,234 B1
• Filed: 02/05/1999
• Issued: 07/01/2003
• Est. Priority Date: 05/02/1997
• Status: Expired due to Term

• Current Assignee: Biomerieux Incorporated (Compagnie Merieux Alliance SAS)
• Original Assignee: Biomerieux Incorporated (Compagnie Merieux Alliance SAS)
• Inventors: Burg, J. Lawrence, Vera-Garcia, Marcela, Moe, James G., Catanzariti, Luigi
• Primary Examiner(s): Sisson, Bradley L.
• Application Number:
  US09/245,940
• Time in Patent Office: 1,607 Days
• Field of Search: 435/6, 435/91.1, 435/91.2, 435/183, 435/287.2, 436/94, 536/23.1, 536/24.3, 536/24.33, 536/25.3
• US Class Current: 435/287.2
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 1/6846 Common amplification features
• CPC Class Codes: C12Q 2527/125 Specific component of sample, medium or buffer
• CPC Class Codes: C12Q 2537/125 Sandwich assay format
• CPC Class Codes: C12Q 2545/101 with an internal standard/control
• CPC Class Codes: C12Q 2565/519 characterised by the capture moiety being a single stranded oligonucleotide
• CPC Class Codes: C12Q 2565/629 being a microfluidic device
• CPC Class Codes: C12Q 2600/166 Oligonucleotides used as internal standards, controls or normalisation probes
• CPC Class Codes: G01N 35/026 having blocks or racks of reaction cells or cuvettes
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

The present invention relates to the detection of specific nucleic acid sequences after an amplification process, or directly without amplification. In particular, the invention provides for the automation of the amplification and detection process, the amplification and detection of one or more specific nucleic acid sequences, the use of internal controls, reduced potential for contamination caused by the manual manipulation of reagents, and improved reagent compositions to better control assay performance and provide for further protection against contamination.

Claims

1. A device for the automated detection of at least two target nucleic acid sequences, said device comprising a pipette-like device having an internal and external surface and an internal cavity defined by said internal surface, wherein said internal surface is coated with at least two capture nucleic acid sequences which bind to said target nucleic acid sequences to form hybridization complexes, and wherein said target nucleic acids are discretely detected in said device.

2. A device as in claim 1 wherein said capture nucleic acids are coated on said internal surface of said device in at least two distinct zones.

3. The device as claimed in claim 2 wherein said zones are spatially separated.

4. The device as in claim 3 wherein said zones are coated homogenously.

5. The device as in claim 2 wherein said zones are coated homogenously.

6. A device as in claim 1 wherein said capture nucleic acids are coated in said internal surface of said device in a single homogenous zone.

7. The device as in claim 1 wherein said capture nucleic acids are coated on said internal surface of said device in two distinct zones.

8. The device as claimed in claim 7 wherein said zones are spatially separated.

9. The device as in claims 1 wherein the internal cavity is coated with a first capture nucleic acid sequence and a second capture nucleic acid sequence, said first capture nucleic acid coated in a first zone and said second capture nucleic acid coated in a second zone of said internal cavity, wherein said first or second capture sequences hybridize to complementary target nucleic acid sequences to form hybridization complexes in said first or second zone.

10. The device as claimed in claim 9 wherein said first and second zones are spatially separated.

11. The device as in claim 1 wherein said target nucleic acids are detected for results selected from the group consisting of truncated gene expression, specific allele and specific spliced genes.