Microchip based enzymatic analysis

• US 6,586,253 B1
• Filed: 04/10/1998
• Issued: 07/01/2003
• Est. Priority Date: 02/27/1998
• Status: Expired due to Fees

• Current Assignee: The Governors of the University of Alberta (University of Alberta)
• Original Assignee: The Governors of the University of Alberta (University of Alberta)
• Inventors: Ocvirk, Gregor, Andersson, Per E., Arriaga, Edgar, Harrison, D. Jed
• Primary Examiner(s): NGUYEN, BAO THUY L
• Application Number:
  US09/058,702
• Time in Patent Office: 1,908 Days
• Field of Search: 422/58, 422/63, 422/93, 422/98, 422/101, 435/7.4, 435/7.6, 435/287.1, 436/518, 436/514
• US Class Current: 435/7.2
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: C12Q 1/34 involving hydrolase
• CPC Class Codes: C12Q 1/48 involving transferase
• CPC Class Codes: C12Q 1/66 involving luciferase
• CPC Class Codes: G01N 1/31 Apparatus therefor
• CPC Class Codes: G01N 2500/10 involving cells
• CPC Class Codes: G01N 2500/20 cell-free systems
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

Abstract

A method for the detection of cell contents, the method comprising the steps of, introducing a cell into a channel in a microchip; lysing the cell to release cell contents into the channel; moving the cell contents towards a detection zone; and detecting the cell contents at the detection zone. An apparatus for the detection of cell contents, the apparatus comprising: a microchip; a cell mobilization channel formed in the microchip, the cell mobilization channel having a cell introduction end and a detection end; a cell mobilizer operably connected with the cell introduction end for moving cells from the cell introduction end to the detection end; means for lysing cells in the cell mobilization channel at a lysing zone, the lysing zone being located between the cell introduction end and the detection end; and a detector, disposed adjacent the detector end, arranged to detect cell contents appearing at the detector end that have been moved from the lysing zone to the detector end by the cell mobilizer.

Claims

1. A method for the detection of cell contents, the method comprising the steps of:

introducing a stream of multiple cells into a channel in a microchip;

individually isolating a cell from the stream of multiple cells in a lysing zone and lysing the cell in the lysing zone to release enzymes into the channel;

reacting the enzymes with a substrate to yield a reaction product;

moving the reaction product towards a detection zone; and

detecting the reaction product at the detection zone.

2. The method of claim 1 in which the enzymes are selected from the group consisting of luciferase, β

-galactosidase and Fucosyltransferase.

3. The method of claim 1 in which the enzymes are produced by reporter genes that have been transfected into the cell.

4. The method of claim 1 in which the cell is lysed by application of an electric field across the cell.

5. The method of claim 1 in which the cell is lysed by introduction of a reagent into the channel.

6. The method of claim 1 in which lysing of the cell is carried out by application of an electric field across the cell assisted by introduction of a reagent.

7. The method of claim 1 in which the channel dimensions are not more than twelve times the cell diameter.

8. The method of claim 1 in which enzyme substrate is introduced into the channel at a location downstream from the introduction of the lysing agent.

9. A method for the detection of cell contents, the method comprising the steps of:

introducing a stream of multiple cells into a channel in a microchip;

individually isolating a cell from the stream of multiple cells in a lysing zone and lysing the cell by application of an electric field across the cell in the lysing zone to release cell contents into the channel;

moving the cell contents towards a detection zone; and

detecting the cell contents at the detection zone.

10. The method of claim 9 in which the cell is a mammalian cell and the cell contents comprise enzymes.

11. The method of claim 10 in which the enzymes are selected from the group consisting of luciferase, β

-galactosidase and Fucosyltransferase.

12. The method of claim 9 in which the enzymes are produced by reporter genes that have been transfected into the cell.

13. The method of claim 9 in which lysing of the cell is carried out by application of an electric field across the cell assisted by introduction of a reagent.

14. The method of claim 9 in which the channel dimensions are not more than twelve times the cell diameter.

15. The method of claim 9 in which, after introducing the cell into a channel, the cell is moved to a lysing zone at an intersection of the channel and a cross-channel, the cell is stopped at the lysing zone and the cell is lysed by application of an electric field applied along the cross-channel.

16. A method for the detection of cell contents, the method comprising the steps of:

introducing a stream of multiple cells into a channel in a microchip;

individually isolating a cell from the stream of multiple cells in a lysing zone and lysing the cell to release cell contents into the channel at the lysing zone;

reacting the cell contents with a substrate at a reaction zone downstream of the lysing zone to yield a reaction product;

moving the reaction product towards a detection zone; and

detecting the reaction product at the detection zone.

17. The method of claim 16 in which the cell is a mammalian cell and the cell contents comprise enzymes.

18. The method of claim 17 in which the enzymes are selected from the group consisting of luciferase, β

-galactosidase and Fucosyltransferase.

19. The method of claim 16 in which the enzymes are produced by reporter genes that have been transfected into the cell.

20. The method of claim 16 in which the cell is lysed by application of an electric field across the cell.

21. The method of claim 16 in which the cell is lysed by introduction of a reagent into the channel.

22. The method of claim 16 in which lysing of the cell is carried out by application of an electric field across the cell assisted by introduction of a reagent.

23. The method of claim 16 in which the channel dimensions are not more than twelve times the cell diameter.

24. A method for the detection of cell contents, the method comprising the steps of:

introducing a stream of multiple cells into a channel in a microchip;

individually isolating a cell from the stream of multiple cells in a lysing zone and lysing the cell by application of an electric field across the cell in the lysing zone to release cell contents into the channel;

reacting the cell contents with a substrate to create reaction products;

moving the reaction products towards a detection zone; and

detecting the reaction products at the detection zone.