Solid support for mass spectrometry

• US 6,589,485 B2
• Filed: 06/11/2001
• Issued: 07/08/2003
• Est. Priority Date: 03/17/1995
• Status: Expired due to Term

• Current Assignee: Agena Bioscience Inc. (Mesa Laboratories Inc.)
• Original Assignee: Sequenom Incorporated (Laboratory Corporation Of America Holdings)
• Inventors: Köster, Hubert
• Primary Examiner(s): Horlick, Kenneth R.
• Application Number:
  US09/879,341
• Publication Number:
  US 20020150903A1
• Time in Patent Office: 757 Days
• Field of Search: 422/104, 435/6, 436/94
• US Class Current: 250/440.11
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/6858 Allele-specific amplification
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 1/6862 Ligase chain reaction [LCR]
• CPC Class Codes: C12Q 1/6872 involving mass spectrometry
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 1/706 for hepatitis
• CPC Class Codes: C12Q 2521/325 Single stranded exonuclease
• CPC Class Codes: C12Q 2523/107 Chemical cleaving agents
• CPC Class Codes: C12Q 2523/319 Photocleavage, photolysis, photoactivation
• CPC Class Codes: C12Q 2525/107 incorporating a peptide nucleic acid
• CPC Class Codes: C12Q 2535/113 Cycle sequencing
• CPC Class Codes: C12Q 2535/125 Allele specific primer extension
• CPC Class Codes: C12Q 2535/131 Allele specific probes
• CPC Class Codes: C12Q 2537/113 Heteroduplex formation
• CPC Class Codes: C12Q 2565/625 being a nucleic acid test strip device, e.g. dipsticks, strips, tapes, CD plates
• CPC Class Codes: C12Q 2565/627 being a mass spectrometer
• CPC Class Codes: G01N 35/1067 for transfer to or from containers having different spacing
• CPC Class Codes: H01J 49/00 : Particle spectrometers or separator tubes
• CPC Class Codes: Y10T 436/143333 : Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/24 : Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Abstract

Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and sequences in the molecules are provided. Arrays of oligonucleotides for performing mass spectrometric analyses are provided. In one aspect, a solid support is provided that includes a plurality of single-stranded oligonucleotides immobilized thereon by a linker cleavable under conditions of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry; and matrix for performing MALDI mass spectrometry, wherein the support comprises a flat surface.

Claims

1. A solid support, comprising:

a plurality of single-stranded oligonucleotides immobilized thereon by a linker cleavable under conditions of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry; and

matrix for performing MALDI mass spectrometry, wherein the support comprises a flat surface.

2. The solid support of claim 1, wherein the oligonucleotides comprise an array.

3. The solid support of claim 1 that is selected from the group consisting of glass fiber filters, glass surfaces and metal surfaces.

4. The solid support of claim 1 that is selected from the group consisting of steel, gold, silver, aluminum, copper and silicon.

5. The solid support of claim 1 that is a silicon.

6. The solid support of claim 1 that is a silicon wafer.

7. The solid support of claim 1, wherein the linker is photocleavable.

8. The solid support of claim 1, wherein the oligonucleotides comprise oligoribonucleotides, oligodeoxyribonucleotides, nucleotide analogs, or peptide nucleic acids (PNA).

9. The solid support of claim 8, wherein the nucleotide analogs comprise a thio-modified phosphodiester or phosphotriester backbone.

10. The support of claim 1, wherein the linker is selected from the group consisting of a heterobifunctional derivative of a trityl ether group, a charge transfer complex and a quaternary ammonium group.

11. The solid support of claim 1, wherein an oligonucleotide spacer separates each oligonucleotide strand from the support.

12. The solid support of claim 11, wherein the oligonucleotide spacer is at least about five nucleotides in length.

13. A solid support, comprising:

a plurality of single-stranded oligonucleotides immobilized thereon by a linker selected from the group consisting of a leuvinyl group, a pyrophosphate bond, and a ribonucleotide bond; and

matrix for performing MALDI mass spectrometry, wherein the support comprises a flat surface.