Measurement and use of molecular interactions
First Claim
1. A method for determining the affinity between first and second binding partners, which comprises the steps of:
- (i) contacting the first and second binding partners, wherein the first binding partner is immobilised on a surface, and wherein the first and second binding partners bind to each other;
(ii) oscillating the surface such that the amplitude of oscillation increases to a value at which the first and second binding partners dissociate; and
(iii) detecting the dissociation of the first and second binding partners, wherein the affinity of the first and second binding partners is determined from the dissociation event.
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Accused Products
Abstract
A method for determining the affinity between binding partners, or a property of one of the binding partners dependent on the affinity, comprising the steps of:
(i) contacting the binding partners, one of which is immobilised on a surface;
(ii) oscillating the surface at increasing amplitude; and
(iii) detecting a dissociation event.
An analogous method can be used to separate a target analyte from a composition. The subject invention also pertains to an apparatus for determining the affinity between binding partners, and comprises: a surface (10) having one binding partner (16) immobilised thereon; means for oscillating the surface at increasing amplitude; and a device (14, 15) for detecting a dissociation event.
90 Citations
35 Claims
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1. A method for determining the affinity between first and second binding partners, which comprises the steps of:
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(i) contacting the first and second binding partners, wherein the first binding partner is immobilised on a surface, and wherein the first and second binding partners bind to each other;
(ii) oscillating the surface such that the amplitude of oscillation increases to a value at which the first and second binding partners dissociate; and
(iii) detecting the dissociation of the first and second binding partners, wherein the affinity of the first and second binding partners is determined from the dissociation event. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
(iv) measuring the voltage at which the disassociation event occurs.
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14. A method for separating a target analyte from a composition, which comprises the steps of:
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(i) contacting the composition with a binding partner for the analyte, the binding partner being immobilised on a surface; and
(ii) oscillating the surface such that the amplitude increases to a value wherein the analyte, or other components of the composition, are selectively removed or dissociated from the surface, thereby separating the target analyte from the composition. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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25. A method for detecting a target analyte in a composition, which comprises the steps of:
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(i) contacting the composition with a binding partner for the analyte, the binding partner being immobilised on a surface; and
(ii) oscillating the surface such that the amplitude increases to a value wherein the analyte dissociates from the binding partner, and wherein detection of the dissociation event is indicative of the target analyte in the composition. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33, 34, 35)
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Specification