Method and apparatus for performing biological reactions on a substrate surface
First Claim
1. An apparatus for performing biological reactions, comprising:
- (a) a glass microscope slide having a first surface and a second surface opposite thereto;
(b) an array of biomolecules positioned on the first surface of the slide, wherein each biomolecule within the array is anchored to the first surface by a polyacrylamide gel pad;
(c) a layer of polyvinylidene chloride affixed to the first surface of the slide by a layer of double-sided adhesive, wherein the adhesive layer is deposited on the first surface of the slide and encloses an area thereupon;
(d) a first and second port extending through the slide from the first surface to the second surface thereof each having a first opening and a second opening, wherein the first opening of each port is provided within the area on the first surface of the slide bounded by the adhesive and covered by the flexible layer and the second opening of each port is provided on the second surface of the slide, and wherein each port is in the shape of a truncated cone having a small-diameter end and a large diameter end, and wherein the small diameter end is the first opening and the large diameter end is the second opening;
(e) a layer of foil tape positioned over the second opening of each port;
(f) a layer of a polyethylene glycol positioned between the first surface of the slide and the layer of polyvinylidene chloride;
(g) a reflective layer positioned between the array and the first substrate surface;
(h) a layer of parylene positioned between the reflective layer and the layer of polyvinylidene chloride; and
(i) a resistive heater.
4 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides an apparatus for performing biological reactions on a substrate layer having a multiplicity of oligonucleotide binding sites disposed thereon. The invention provides a hybridization chamber wherein nucleic acid hybridization is performed by reacting biological material on a biochip comprising a substrate having an array of oligonucleotide binding sites. The binding sites are associated with an array of 3-dimensional polyacrylamide pads for anchoring the reactants. The arrays are covered with a flexible layer that permits mixing of the hybridization solution on the biochip and detection of hybridization in situ. Fluid inlet and outlet ports in the chamber provide for control of fluid flow into and out of the chamber.
-
Citations
23 Claims
-
1. An apparatus for performing biological reactions, comprising:
-
(a) a glass microscope slide having a first surface and a second surface opposite thereto;
(b) an array of biomolecules positioned on the first surface of the slide, wherein each biomolecule within the array is anchored to the first surface by a polyacrylamide gel pad;
(c) a layer of polyvinylidene chloride affixed to the first surface of the slide by a layer of double-sided adhesive, wherein the adhesive layer is deposited on the first surface of the slide and encloses an area thereupon;
(d) a first and second port extending through the slide from the first surface to the second surface thereof each having a first opening and a second opening, wherein the first opening of each port is provided within the area on the first surface of the slide bounded by the adhesive and covered by the flexible layer and the second opening of each port is provided on the second surface of the slide, and wherein each port is in the shape of a truncated cone having a small-diameter end and a large diameter end, and wherein the small diameter end is the first opening and the large diameter end is the second opening;
(e) a layer of foil tape positioned over the second opening of each port;
(f) a layer of a polyethylene glycol positioned between the first surface of the slide and the layer of polyvinylidene chloride;
(g) a reflective layer positioned between the array and the first substrate surface;
(h) a layer of parylene positioned between the reflective layer and the layer of polyvinylidene chloride; and
(i) a resistive heater.
-
-
2. An apparatus for performing biological reactions, comprising:
-
(a) a glass microscope slide having a first surface and a second surface opposite thereto, (b) an array of biomolecules positioned on the first surface of the slide, wherein each biomolecule within the array is anchored to the first surface by a polyacrylamide gel pad;
(c) a layer of polyvinylidene chloride affixed to the first surface of the slide by a layer of double-sided adhesive, wherein the adhesive layer is deposited on the first surface of the slide and encloses an area thereupon;
(d) a first and second port extending through the slide from the first surface to the second surface thereof each having a first opening and a second opening, wherein the first opening of each port is provided within the area on the first surface of the slide bounded by the adhesive and covered by the flexible layer and the second opening of each port is provided on the second surface of the slide, and wherein each port is in the shape of a truncated cone having a small-diameter end and a large diameter end, and wherein the small diameter end is the first opening and the large diameter end is the second opening;
(e) a layer of foil tape positioned over the second opening of each port;
(f) a layer of a polyethylene glycol positioned between the first surface of the slide and the layer of polyvinylidene chloride;
(g) a reflective layer positioned between the array and the first substrate surface;
(h) a layer of parylene positioned between the reflective layer and the layer of polyvinylidene chloride;
(i) a resistive heater;
a case having a lid and a base, wherein the lid further comprises a display unit;
(k) a cavity disposed in the base; and
(l) a carriage comprising a scanner and a roller, wherein the carriage is provided in the cavity, and wherein the slide is removably positioned above the carriage such that the first slide surface is in operative contact with the carriage.
-
-
3. An apparatus for performing biological reactions comprising:
-
a) a substrate comprising a first and a second surface;
b) an array of biomolecular probes positioned on said first surface;
c) a flexible layer comprising a gas permeable membrane affixed to said first surface by an adhesive layer, forming a reaction volume; and
d) a port extending through said substrate from said second surface to said first surface. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
-
-
4. An apparatus for performing biological reactions comprising:
-
a) a substrate comprising a first and a second surface;
b) an array of biomolecular probes positioned on said first surface;
c) a flexible layer affixed to said first surface by an adhesive layer, forming a reaction volume;
d) a port extending through said substrate from said second surface to said first surface;
e) a sample preparation chip; and
wherein said port is a first port that extends from said second surface to said reaction volume and said sample preparation chip is in contact with said second surface and wherein said sample preparation chip has a port that is aligned with said first port.
-
-
5. An apparatus for performing biological reactions comprising:
-
a) a substrate comprising a first and a second surface;
b) an array of biomolecular probes positioned on said first surface;
c) a flexible layer affixed to said first surface by an adhesive layer, forming a reaction volume; and
d) a port extending through said substrate from said second surface to said first surface; and
e) a roller, wherein said roller is in contact with said flexible layer.
-
-
6. A method of making an apparatus comprising:
-
a) providing a substrate with a first and a second surface, wherein said first surface comprises a continuous layer of derivatized polyacrylamide;
b) contacting said derivatized polyacrylamide with biomolecular probes;
c) forming an amide bond between said derivatized polyacrylamide and said biomolecular probe;
d) adding a flexible layer comprising a gas permeable membrane to said first surface to form a reaction volume.
-
-
7. A method of detecting the presence of a target molecule in a sample comprising:
-
a) providing an apparatus comprising;
i) a substrate comprising a first and a second surface;
ii) an array of biomolecular probes positioned on said first surface; and
iii) a flexible layer comprising a gas permeable membrane affixed to said first surface by an adhesive layer, forming a reaction volume;
wherein said apparatus comprises at least a first port into said reaction volume;
b) introducing said sample through said first port into said reaction volume under conditions that allow the binding of said target molecule to at least one of said biomolecular probes; and
c) detecting said binding as an indication of the presence of said target molecule. - View Dependent Claims (21, 22, 23)
-
Specification