Method and apparatus for verifying uniform flow of a fluid sample through a flow cell and distribution on a slide

• US 6,590,646 B2
• Filed: 05/20/2002
• Issued: 07/08/2003
• Est. Priority Date: 05/15/1996
• Status: Expired due to Term

• Current Assignee: IRIS International Inc. (Danaher Corporation)
• Original Assignee: IRIS International Inc. (Danaher Corporation)
• Inventors: Kasdan, Harvey, Ko, Jaehyl
• Primary Examiner(s): Pham, Hoa Q.
• Application Number:
  US10/152,651
• Publication Number:
  US 20030002037A1
• Time in Patent Office: 414 Days
• Field of Search: 356/39, 356/73, 356/246, 356/335-343, 382/199, 382/203, 382/201, 382/204, 382/205, 377/10, 377/11, 436/8, 436/10, 436/16, 250/573, 250/574, 250/576, 738/610.5, 738/611.1
• US Class Current: 356/246
• CPC Class Codes: G01N 15/147 the analysis being performed on a sample stream

Abstract

Particles in a fluid are counted as the fluid flows through an examination area of a particle analyzer. Parameters based upon the particle count are calculated. It is determined whether the at least one calculated parameter is within an allowable range for the parameter. Based on this determination, an indication is provided of uniformity of the fluid sample in the examination area.

Claims

1. A method of determining flow quality of a fluid sample, in a particle analyzer, through an examination area of a flow chamber, comprising:

providing a heavy stain as at least a portion of the fluid sample;

determining, by the particle analyzer, if there is a period of time during which a stained portion of the fluid sample passes through the examination area after the providing step and is uniformly distributed within a first portion of the examination area, to generate a determination signal; and

determining the flow quality of the fluid sample in response to the determination signal.

2. The method of claim 1, wherein the first portion of the examination area is a portion of the examination area which spans the examination area in a direction perpendicular to a direction of flow of the fluid sample through the examination area.

3. The method of claim 1, wherein the providing step is executed by the particle analyzer concurrently with the particle analyzer analyzing particles in a fluid sample.

4. The method of claim 1, wherein the providing step is executed by the particle analyzer at a time when the particle analyzer is not analyzing particles in a fluid sample.

5. The method of claim 1, wherein the particle analyzer includes a light source illuminating the examination area from a first side and an imaging source imaging the examination area from a second side, opposite the first side, to generate a plurality of images, and

wherein, in the determining step, the particle analyzer determines from the images if there is a period of time during which a stained portion of the fluid sample passes through the examination area after the providing step and is uniformly distributed within the first portion of the examination area.

6. The method of claim 5, wherein the first portion of the examination area is a portion of the examination area which spans the examination area in a direction perpendicular to a direction of flow of the fluid sample through the examination area.

7. The method of claim 1, wherein the at least one parameter includes a weighted sum of the particle counts.