Microdialysis system

• US 6,591,126 B2
• Filed: 07/20/2001
• Issued: 07/08/2003
• Est. Priority Date: 08/04/2000
• Status: Expired due to Fees

• Current Assignee: Roche Diabetes Care Inc. (Roche Holding AG)
• Original Assignee: Roche Diagnostics Corporation (Roche Holding AG)
• Inventors: Hoerauf, Christian, Schoemaker, Michael, Roeper, Josef
• Primary Examiner(s): JACYNA, J CASIMER
• Application Number:
  US09/910,452
• Publication Number:
  US 20020082490A1
• Time in Patent Office: 718 Days
• Field of Search: 600/345, 600/347, 600/352, 600/365, 600/366, 604/19
• US Class Current: 600/347
• CPC Class Codes: A61B 5/14528 invasively
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement

Abstract

The invention concerns a microdialysis system comprising a microdialysis probe (12) that can be inserted into organic tissue (10) and has a dialysis membrane (28) to separate a probe channel (18) filled with perfusion fluid from the tissue (10), a sensor cell (14) to determine the concentration of components and especially glucose in the perfusion fluid that is conveyed from the microdialysis probe (12) and a transport device (16) to convey the perfusion fluid through the probe channel (18) of the microdialysis probe (12) to the sensor cell (14). In order to enable a substantially pressureless transport of perfusion fluid through the microdialysis probe and thus to prevent undesired passage of fluid through the dialysis membrane (28), it is proposed that the transport device (16) has a pressure pump unit (30) connected on the pressure side to the inlet (20) of the probe channel (18) and a suction pump unit (31) connected on the suction side to the outlet (24) of the probe channel (18) that can be operated simultaneously with the pressure pump unit (30).

Claims

1. Microdialysis system comprising

a microdialysis probe (12) that can be inserted in organic tissue (10) and has a dialysis membrane (28) to separate a probe channel (18) from the tissue (10),

a sensor cell (14) for the detection of components in the perfusion fluid that is conveyed from the microdialysis probe (12) and

a transport device (16) to convey a perfusion fluid through the probe channel (18) of the microdialysis probe (12) to the sensor cell (14), characterized in that the transport device (16) has a pressure pump unit (30) connected on a pressure side to an inlet (20) of the probe channel (18) and a suction pump unit (31) having a suction side and being connected on the suction side to an outlet (24) of the probe channel (18) the suction pump unit operating simultaneously with the pressure pump unit (30).

2. Microdialysis system as claimed in claim 1, characterized in that the pressure side of the pressure pump unit (30) is exclusively connected to the inlet (20) and the suction side of the suction pump unit (31) is exclusively connected to the outlet (24) of the probe channel (18).

3. Microdialysis system as claimed in claim 1, characterized in that the pressure pump unit (30) and the suction pump unit (31) are each connected via a non-branching duct (36, 38) comprising a flexible tube, to the probe channel (18) of the microdialysis probe.

4. Microdialysis system as claimed in claim 1, characterized in that the delivery rates of the pressure and suction pump unit (30, 31) are matched in order to reduce the effective pressure difference across the dialysis membrane (28) between the perfusion and tissue fluid.

5. Microdialysis system as claimed in claim 1, characterized in that the delivery rates of the pressure and suction pump unit (30, 31) are essentially equal.

6. Microdialysis system as claimed in claim 1, characterized in that the delivery rate of the pressure pump unit (30) is larger by a predetermined amount than the delivery rate of the suction pump unit (31).

7. Microdialysis system as claimed in claim 1, characterized in that the delivery rate of the pressure and suction pump unit (30, 31) is less than 1 μ

l per minute.

8. Microdialysis system as claimed in claim 1, characterized in that the suction side of the pressure pump unit (30) is connected to a reservoir (34) for perfusion fluid.

9. Microdialysis system as claimed in claim 1, characterized in that the pressure side of the suction pump unit (31) is connected to a flow chamber (25) of the sensor cell (14) which terminates in a collecting vessel (54).

10. Microdialysis system as claimed in claim 1, characterized by a reagent pump unit (44) for metering a reagent solution into the perfusion fluid upstream of the sensor cell.

11. Microdialysis system as claimed in claim 10, characterized in that the pressure side of the reagent pump unit (44) and of the suction pump unit (31) are connected to a connecting duct (42) leading to the sensor cell (14).

12. Microdialysis system as claimed in claim 10, characterized in that the pressure pump unit (30), the suction pump unit (31) and the reagent pump unit (44) each comprise a flexible pump tube of a multichannel peristaltic pump actuated by a common rotary piston (50).

13. Microdialysis system as claimed in claim 1, characterized in that the delivery rate of the pressure and suction pump unit (30, 31) is less than 0.1 μ

l per minute.

14. Microdialysis system as claimed in claim 1, characterized in that the sensor cell is formed for the detection of glucose in the perfusion fluid.

15. Microdialysis system as claimed in one of the claims 1 to 12, characterized in that the microdialysis probe (12) comprises a double-lumen catheter (22, 26) which has in the area of the catheter'"'"'s distal end the micropore dialysis membrane (28) formed from hollow fibres the outside of which is formed to be embedded in the tissue (10) and the inside of which is supplied with perfusion fluid.

16. A microdialysis system comprising

a microdialysis probe formed to be inserted in organic tissue to collect fluid from the tissue, the probe defining a probe channel having an inlet and an outlet and including a dialysis membrane, the dialysis membrane being formed to permit diffusion of fluid into the channel and to separate the probe channel from the surrounding tissue,

a sensor cell, and

a transport device formed to convey the fluid from the microdialysis probe to the sensor cell, the transport device including a pressure pump unit in communication with the inlet and a suction pump unit having a pressure side and a suction side and the suction side is in communication with the outlet, the pressure and suction pump units being formed to operate simultaneously with one another.

17. The system of claim 16 wherein the pressure pump unit includes a pressure side in communication with the inlet.

18. The system of claim 16 wherein the pressure side is in communication with the sensor cell.

19. The system of claim 17 wherein the sensor cell includes a flow chamber that terminates in a collecting vessel and the pressure side of the suction pump unit is in communication with the flow chamber.

20. The system of claim 16 further comprising a reservoir for perfusion fluid and wherein the pressure pump unit includes a suction side in communication with the reservoir.

21. The system of claim 16 wherein the pressure pump unit and the suction pump unit are each coupled via a non-branching duct to the probe channel.

22. The system of claim 21 wherein the duct is a flexible tube.

23. The system of claim 16 wherein pressure pump unit and the suction pump unit each include a delivery rate and the delivery rates are essentially equal.

24. The system of claim 16 wherein pressure pump unit and the suction pump unit each include a delivery rate and the delivery rate of the pressure pump unit is larger by a predetermined amount than the delivery rate of the suction pump unit.

25. The system of claim 16 wherein the pressure pump unit and the suction pump unit each include a delivery rate and the delivery rates are each less than 1 μ

l per minute.

26. The system of claim 25 wherein the delivery rates are each less than 0.1 μ

l per minute.

27. The system of claim 16 wherein the pressure pump unit and the suction pump unit each comprise a flexible pump tube of a multichannel peristaltic pump actuated by a common rotary piston.

28. The system of claim 16 wherein the microdialysis probe comprises a double-lumen catheter.

29. The system of claim 28 wherein the catheter has at an area of the catheter'"'"'s distal end the micropore dialysis membrane.

30. The system of claim 29 wherein the membrane is formed of a hollow fibre.

31. The system of claim 30 wherein the outside of the fibre is formed to be embedded in the tissue and the inside of the fibre is formed to be supplied with the fluid.

32. A microdialysis system comprising

a microdialysis probe formed to be inserted in organic tissue to collect fluid from the tissue, the probe defining a probe channel having an inlet and an outlet and including a dialysis membrane, the dialysis membrane being formed to permit diffusion of fluid into the channel and to separate the probe channel from the surrounding tissue,

a sensor cell,

a transport device formed to convey the fluid from the microdialysis probe to the sensor cell, the transport device including a pressure pump unit in communication with the inlet and a suction pump unit in communication with the outlet, the pressure and suction pump units being formed to operate simultaneously with one another, and

a reagent pump unit formed to meter a reagent solution into the fluid upstream of the sensor cell.

33. The system of claim 32 wherein the reagent solution is an enzyme solution.

34. The system of claim 32 wherein the reagent pump unit and the suction pump unit are coupled to a duct leading to the sensor cell.

35. The system of claim 34 wherein the reagent pump unit includes a pressure side that is coupled to the duct.

36. The system of claim 34 wherein the duct is coupled to a Y-shaped connecting piece.

37. The system of claim 32 wherein the pressure pump unit, the suction pump unit, and the reagent pump unit each comprise a flexible pump tube of a multichannel peristaltic pump actuated by a common rotary piston.

38. A microdialysis system comprising

a microdialysis probe formed to be inserted in organic tissue to collect fluid from the tissue, the probe defining a probe channel having an inlet and an outlet and including a dialysis membrane, the dialysis membrane being formed to permit diffusion of fluid into the channel and to separate the probe channel from the surrounding tissue,

a sensor cell including a flow chamber,

a reservoir for perfusion fluid, and

a transport device formed to convey the fluid from the microdialysis probe to the flow chamber of the sensor cell, the transport device including a pressure pump unit having a pressure side in communication with the inlet and a suction side in communication with the reservoir and a suction pump unit having a suction side in communication with the outlet and a pressure side in communication with the flow chamber of the sensor cell, the pressure and suction pump units being formed to operate simultaneously with one another.

39. The system of claim 38 wherein the pressure pump unit and the suction pump unit are each coupled via a non-branching duct to the probe channel.

40. The system of claim 38 wherein pressure pump unit and the suction pump unit each include a delivery rate and the delivery rates are essentially equal.

41. The system of claim 38 wherein pressure pump unit and the suction pump unit include a delivery rate and the delivery rate of the pressure pump unit is larger by a predetermined amount than the delivery rate of the suction pump unit.

42. The system of claim 38 wherein the pressure pump unit and the suction pump unit each include a delivery rate and the delivery rates are each less than 1 μ

l per minute.

43. A The system of claim 42 wherein the delivery rates are each less than 0.1 μ

l per minute.

44. The system of claim 38 further comprising a reagent pump unit formed to meter a reagent solution into the fluid upstream of the sensor cell.

45. The system of claim 44 wherein the pressure pump unit, the suction pump unit, and the reagent pump unit each comprise a flexible pump tube of a multichannel peristaltic pump actuated by a common rotary piston.

46. The system of claim 38 wherein the microdialysis probe comprises a double-lumen catheter.