Tenascin-C nucleic acid ligands
First Claim
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1. A complex for use in in vivo diagnostics comprising a tenascin-C nucleic acid ligand and a marker.
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Abstract
Methods are described for the identification and preparation of nucleic acid ligands to tenascin-C. Included in the invention are specific RNA ligands to tenascin-C identified by the SELEX method. Further included in the invention are methods for detecting the presence of a disease condition in a biological tissue in which tenascin-C is expressed.
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18 Claims
- 1. A complex for use in in vivo diagnostics comprising a tenascin-C nucleic acid ligand and a marker.
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12. A method for the preparation of a complex comprised of a tenascin-C nucleic acid ligand and a marker, said method comprising:
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a) contacting a candidate mixture of nucleic acids with tenascin-C, wherein nucleic acids having an increased affinity to tenascin-C relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
b) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture;
c) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with relatively higher affinity and specificity for binding to tenascin-C to yield a tenascin-C nucleic acid ligand; and
d) covalently linking said tenascin-C nucleic acid ligand with a marker. - View Dependent Claims (13, 14, 15, 16, 17, 18)
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Specification