Threshold glucose detection in urine

• US 6,599,474 B2
• Filed: 08/30/2002
• Issued: 07/29/2003
• Est. Priority Date: 06/18/2001
• Status: Expired due to Term

• Current Assignee: Societe des Produits Nestle SA (Nestle SA)
• Original Assignee: Nestec SA (Nestle SA), Environmental Test Systems, Inc. (Veralto Corp.)
• Inventors: Lewis, Ronald A. II, Dobler, Lydia, Van Lente, Michael A., Evtodienko, Iouri, Evtodienko, Vladimir
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): SIEFKE, SAMUEL P
• Application Number:
  US10/232,123
• Publication Number:
  US 20030022385A1
• Time in Patent Office: 333 Days
• Field of Search: 435/15, 435/10, 422/56, 422/57, 422/61, 422/68.1, 436/95, 436/169
• US Class Current: 436/95
• CPC Class Codes: G01N 33/523 the element being adapted for a specific analyte
• CPC Class Codes: Y10T 436/144444 Glucose

Abstract

The present invention is directed to a reagent composition for use in detection and quantification of glucose in urine comprising a first chromogenic indicator reagent combination capable of color development to indicate the presence of a lower concentration of glucose, a second chromogenic indicator reagent combination capable of color development to indicate the presence of a higher concentration of glucose, wherein the first chromogenic indicator prevents color development of the second chromogenic indicator unless the higher concentration of glucose is present, a scavenger that prevents color development of the first indicator reagent composition unless a threshold concentration of glucose is present.

Claims

1. A reagent composition for use in detection and quantification of glucose in urine comprising:

a first chromogenic indicator reagent combination capable of color development to indicate the presence of a lower concentration of glucose, and

a second chromogenic indicator reagent combination capable of color development to indicate the presence of a higher concentration of glucose, wherein the first chromogenic indicator prevents color development of the second chromogenic indicator unless the higher concentration of glucose is present, and

a scavenger that prevents color development of the first indicator reagent composition unless a threshold concentration of glucose is present.

2. The composition of claim 1 wherein the scavenger comprises cysteine.

3. The composition of claim 1 wherein the scavenger is selected from the group consisting of thiosulfates and mercaptans.

4. The composition of claim 1 wherein the threshold glucose concentration is about 50 mg/dL.

5. The dev composition ice of claim 4 wherein the lower concentration of glucose is in the range of 150-300 mg/dL.

6. The composition of claim 5 wherein the higher concentration of glucose is 300 mg/dL and the second indicator is capable of color development to indicate concentrations of glucose in the range of 300-600 mg/dL.

7. The composition of claim 1 wherein the first indicator comprises 4-aminoantipyrene and a phenolic compound.

8. The composition of claim 7 wherein the phenolic compound is selected from the group consisting of 2-hydroxy-3,5-dichlorobenzenesulfonate, 3-methylcatechol, 4-hydroxybenzenesulfonic acid, and 2,6-dimethylphenol.

9. The composition of claim 1 wherein the second indicator comprises an iodide salt.

10. The composition of claim 9 wherein the iodide salt is potassium iodide and the second indicator further comprises starch.

11. The composition of claim 1 wherein a resultant color obtained from the color development is independent of the specific gravity of the urine.