Array fabrication

• US 6,599,693 B1
• Filed: 07/31/2000
• Issued: 07/29/2003
• Est. Priority Date: 07/31/2000
• Status: Expired due to Fees

• Current Assignee: Agilent Technologies Europe BV (Agilent Technologies, Inc.)
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Webb, Peter G.
• Primary Examiner(s): Wang, Andrew
• Assistant Examiner(s): FRIEND, TOMAS H F
• Application Number:
  US09/628,963
• Time in Patent Office: 1,093 Days
• Field of Search: 422/81, 436/518, 436/43, 436/63, 436/86, 436/94, 530/300, 435/41, 435/4, 435/6, 435/7.1, 435/DIG.49
• US Class Current: 506/16
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

A method of fabricating an array with multiple sets of neighboring features. In the method, for each of multiple sets of neighboring features, at least one set of drops is deposited from a corresponding same pulse jet dispenser onto a substrate so as to form the array with the sets formed from drops deposited by respective different dispensers. Apparatus and computer program products which can execute a method of the invention, are also provided.

Claims

1. A method of fabricating an array with multiple sets of neighboring features comprising, for each of multiple sets of neighboring features, depositing at least one set of drops from a same corresponding pulse jet dispenser onto a substrate, at least some drops in a set of drops containing different biopolymers from one another, so as to form the array with different sets of features formed from drops deposited by respective different dispensers, the features of each same set being formed by drops deposited by a same dispenser.

2. A method according to claim 1 wherein the different dispensers are moved in unison with respect to the substrate during deposition of respective sets of drops from the different dispensers.

3. A method according to claim 2 wherein the different dispensers deposit one or more of the drops of their respective sets on a same pass over the substrate.

4. A method according to claim 1 wherein each feature set has at least ten features with ten different biopolymers.

5. A method according to claim 1 wherein features within each of multiple feature sets comprise polynucleotides or peptides.

6. A method according to claim 5 wherein features within each of multiple feature sets comprise polynucleotides.

7. A method according to claim 5 wherein features within each of multiple feature sets comprise peptides.

8. A method according to claim 1 wherein at least ten different dispensers are used.

9. A method according to claim 1 wherein each set of neighboring features includes at least four features in a non-linear configuration.

10. A method according to claim 1 wherein a distance between at least two neighboring sets is greater than an average distance between features within the sets, both as measured in a same direction.

11. A method of fabricating multiple arrays, comprising fabricating the arrays on a same substrate each according to claim 1, the method additionally comprising separating the substrate into multiple segments each carrying at least one of the arrays.

12. A method according to claim 1 wherein the distance between at least two neighboring sets of features is no greater than 2 mm.

13. A method according to claim 1 wherein the sets of features are formed from drops deposited by respective different dispensers on a same deposition head.

14. A method according to claim 13 wherein the deposition head has at least ten dispensers.

15. A method according to claim 13 wherein the deposition head has at least twenty different dispensers.

16. A method according to claim 1 wherein the sets of features are formed from drops deposited by respective ones of at least ten dispensers on a same deposition head, which different dispensers of the same head comprise different orifices on a same one-piece member.

17. A method according to claim 1 wherein the sets of features are formed from drops deposited by respective ones of at least twenty dispensers on a same deposition head, which different dispensers of the same head comprise different orifices on a same one-piece member.

18. A method according to claim 1 wherein the sets of features are formed from drops deposited by respective ones of at least one hundred dispensers on a same deposition head, which different dispensers of the same head comprise different orifices on a same one-piece member.

19. A method of fabricating an array with multiple sets of neighboring features comprising, for each of multiple sets of neighboring features, depositing at least one set of drops from a same corresponding dispenser onto a substrate, at least some drops in a set of drops containing different biopolymers from one another, so as to form the array with different sets of features formed from drops deposited by respective different dispensers, the features of each same set being formed by drops deposited by a same dispenser, wherein a distance between at least two neighboring sets of features is greater than an average distance between features within the sets, both as measured in a same direction.