Electronic methods for the detection of analytes utilizing monolayers

• US 6,600,026 B1
• Filed: 05/06/1999
• Issued: 07/29/2003
• Est. Priority Date: 05/06/1998
• Status: Expired due to Term

• Current Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Original Assignee: Clinical Micro Sensors Inc. (Roche Holding AG)
• Inventors: Yu, Changjun
• Primary Examiner(s): Riley, Jezia
• Application Number:
  US09/306,653
• Time in Patent Office: 1,545 Days
• Field of Search: 536/4.1, 536/17.1, 536/26.26, 536/27.1, 536/28.1
• US Class Current: 536/17.1
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 2610/00 Assays involving self-assembled monolayers [SAMs]
• CPC Class Codes: G01N 27/3277 being a redox reaction, e.g. detection by cyclic voltammetry voltammetry per se G01N27/42, G01N27/48
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

The present invention relates to the use of self-assembled monolayers with mixtures of conductive oligomers and insulators to detect target analytes.

Claims

1. A modified nucleoside having the formula:

4. A modified nucleoside according to claim 1, 2, or 3, wherein said base is selected from the group consisting of adenine, thymine, cytosine, guanine, and uracil.

7. A nucleic acid comprising at least one modified nucleoside according to claim 1.

8. A nucleic acid comprising a plurality of modified nucleosides according to claim 1.

9. A nucleic acid comprising at least four modified nucleosides according to claim 1, wherein said modified nucleosides are separated by at least one unmodified nucleoside.

10. A nucleic acid comprising at least six modified nucleosides according to claim 1 wherein said modified nucleosides are separated by at least one unmodified nucleoside.

11. A nucleic acid comprising at least ten modified nucleosides according to claim 1 wherein said modified nucleosides are separated by at least one unmodified nucleoside.

12. A method of making a nucleic acid by incorporating at least one phosphoramidite modified nucleoside comprising:

a) providing a modified nucleoside according to claim 1;

b) converting said modified nucleoside into a phosphoramidite modified nucleoside;

c) adding said phosphoramidite modified nucleoside to a growing nucleic acid, wherein said nucleic acid is attached to a solid oligomeric support.

2. A modified nucleoside having the formula:

wherein PG is a protecting group.

3. A modified nucleoside having the formula:

wherein PG is a protecting group.

5. A phosphoramidite modified nucleoside having the formula:

wherein PG is a protecting group.

6. A phosphoramidite modified nucleoside according to claim 5, wherein said base is selected from the group consisting of adenine, thymine, cytosine, guanine, and uracil.