Coupling to microstructures for a laboratory microchip

• US 6,602,472 B1
• Filed: 05/15/2000
• Issued: 08/05/2003
• Est. Priority Date: 10/01/1999
• Status: Expired due to Term

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Zimmermann, Hans-Peter, Plé, Gerhard
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): SINES, BRIAN J
• Application Number:
  US09/570,948
• Time in Patent Office: 1,177 Days
• Field of Search: 422/100, 422/101, 422/102, 422/103, 264/34, 216/52, 438/940, 205/701, 435/6, 204/450, 204/451, 204/600
• US Class Current: 422/508
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0622 distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
• CPC Class Codes: B01L 2400/0633 with moving parts
• CPC Class Codes: B01L 3/0241 Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: G01N 2035/00247 Microvalves
• CPC Class Codes: G01N 27/44717 Arrangements for investigating the separated zones, e.g. localising zones
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: H01J 49/04 Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components

Abstract

A laboratory microchip providing a microspray has a substrate having a channel structure on one side thereof. A microspray tip is on the other side of the substrate close to an edge of the substrate. The substrate is deformable in a particular region. Deformation results from thinning the substrate locally in the region or by providing a linear perforation that extends approximately perpendicular to the plane of the substrate. Alternatively, deformation can be obtained by selecting a suitable material for the entire substrate. Substrate deformation can be permanent or reversible.

Claims

1. A microfluidic microchip for chemical, physical, and/or biological analysis or synthesis of substances comprising a deformable substrate carrying a channel structure, the channel structure being arranged so the substances can be moved to follow the channel structure by applying an electrical potential to the channel structure, a portion of the channel structure being on a deformable section of the substrate that is thinner than nondeformable sections of the substrate.

2. A microfluidic microchip for chemical, physical, and/or biological analysis or synthesis of substances comprising a deformable substrate carrying a channel structure, the channel structure being arranged so the substances can be moved to follow the channel structure by applying an electrical potential to the channel structure, a portion of the channel structure being on a deformable section of the substrate that is thinned.

3. A microfluidic microchip for chemical, physical, and/or biological analysis or synthesis of substances comprising a deformable substrate carrying a channel structure, the channel structure being arranged so the substances can be moved to follow the channel structure by applying an electrical potential to the channel structure, a portion of the channel structure being on a deformable section of the substrate that is perforated.

4. A microfluidic microchip for chemical, physical, and/or biological analysis or synthesis of substances comprising a deformable substrate carrying a channel structure, the channel structure being arranged so the substances can be moved to follow the channel structure by applying an electrical potential to the channel structure, a portion of the channel structure being on a deformable section of the substrate that is microperforated.

5. A microfluidic microchip for chemical, physical, and/or biological analysis or synthesis of substances comprising a deformable substrate carrying a channel structure, the channel structure being arranged so the substances can be moved to follow the channel structure by applying an electrical potential to the channel structure, a portion of the channel structure being on a deformable section of the substrate that includes a bendable tubular duct.

6. The microchip of claim 5 wherein the bendable tubular duct includes a suction pipe to draw in substances to be handled.

7. The microchip of claim 5 wherein the duct is metallized along its entire length.

8. A microfluidic microchip for chemical, physical, and/or biological analysis or synthesis of substances comprising a deformable substrate carrying a channel structure, the channel structure being arranged so the substances can be moved to follow the channel structure by applying an electrical potential to the channel structure, a portion of the channel structure being on a deformable section of the substrate that includes a microspray tip having a substance-conveying connection to at least one channel in the channel.

9. The microchip of claim 8 wherein the microspray tip is metallized along its entire length.

10. A microfluidic microchip for chemical, physical, and/or biological analysis of substances comprising a deformable substrate carrying a channel structure, the channel structure being arranged so the substances can be moved to follow the channel structure by applying an electrical potential to the channel structure, a portion of the channel structure being on a deformable section of the substrate, a detector for sensing the presence of the substances in the channel structure, the detector including a radiation source, a radiation receiver and a detection window for passing radiation from the source, the window being between the source and the receiver and on the substrate in the region of one of the channels of the channel structure, and the substances being feedable to the microchip from one side of the substrate by a sample feed-in system, the region of the substrate including the detection window being located on a deformable section of the substrate that can be bent to one side of the substrate so that the radiation source and the radiation receiver on said one side of the substrate are positioned on opposite sides of the detection window, the sample feed-in being arranged so samples can be fed in from the other side of the substrate.

11. The microchip of claim 10, wherein the sample feed-in includes a bendable suction tube.

12. A microfluidic microchip for chemical, physical, and/or biological analysis or synthesis of substances comprising a deformable substrate carrying a channel structure, the channel structure being arranged so the substances can be moved to follow the channel structure by applying an electrical potential to the channel structure, a portion of the channel structure being on a deformable section of the substrate, the substrate including a sprayer coupled with a deformable section of the substrate carrying the channel structure, the sprayer being arranged for spraying the substances away from the substrate into a mass spectrometer, the substrate being deformable in the region of the sprayer so that the orientation in space of the sprayer is variable about at least one axis of rotation.

13. A microfluidic microchip for chemical, physical, and/or biological analysis of substances comprising a deformable substrate carrying a channel structure, the channel structure being arranged so the substances can be moved to follow the channel structure by applying an electrical potential to the channel structure, a portion of the channel structure being on a deformable section of the substrate, first and second sprayers coupled with the channel structure for spraying the substances into an externally positioned detector, the sprayers including at least one diaphragm, the first and second sprayers being movable next to one another relative to the diaphragm.

14. A microfluidic microchip for chemical, physical, and/or biological analysis of an analyte comprising a substrate carrying a channel structure, the channel structure being arranged so the analyte can be moved to follow the channel structure by applying a potential to the channel structure, first and second portions of the channel structure being respectively parts of first and second sections of the substrate, the first section channel structure being at a non-zero angle with respect to the second section channel structure, the first section channel structure being arranged to be responsive to the analyte, the second section channel structure including a segment for delivering a substance to an analyzer of the substance, the substance in the segment including at least part of the analyte, the segment for delivering at least one substance including a spray tip for spraying the substance into the analyzer.

15. The microchip of claim 14 wherein the channel structure of the first section and the segment for delivering are in different planes, the spray tip having an axis in a plane different from the plane of the segment.

16. The microchip of claim 14 wherein the channel structure of the first section and the segment for delivering are in different planes, the spray tip having an axis in the same plane as the plane of the segment.

17. A microfluidic microchip for chemical, physical, and/or biological analysis of an analyte comprising a substrate carrying a channel structure, the channel structure being arranged so the analyte can be moved to follow the channel structure by applying a potential to the channel structure, first and second portions of the channel structure being respectively parts of first and second sections of the substrate, the first section channel structure being at a non-zero angle with respect to the second section channel structure, the first section channel structure being arranged to be responsive to the analyte, the second section channel structure including a segment or delivering a substance to an analyzer of the substance, the substance in the segment including at least part of the analyte, the segment for delivering and the second portion being pivotable relative to a channel segment in the first portion, the channel segment in the first portion being arranged for supplying the analyte to the channel structure of the second portion, wherein the first and second sections are pivotable about a point in a plane including the channel segment in the first portion, the channel segment in the first portion being arranged so the analyte flows past the point, and wherein the second section channel structure includes a plurality of segments for selectively delivering to one substance sump the substance in said channel segment in the first portion, the plurality of segments being pivotable together about the point.

18. The microchip of claim 17 wherein the segment of the second section is arranged for delivering the substance to plural substance sumps locating at different angles with respect to said channel segment in the first portion.

19. The microchip of claim 17 wherein each of the plural segments of the second channel structure includes a spray tip for spraying the substance in the particular segment into the analyzer.

20. A microfluidic microchip for chemical, physical, and/or biological analysis of an analyte comprising a substrate carrying a channel structure, the channel structure being arranged so the analyte can be moved to follow the channel structure by applying a potential to the channel structure, first and second portions of the channel structure being respectively parts of first and second sections of the substrate, the first section channel structure being at a non-zero angle with respect to the second section channel structure, the first section channel structure being arranged to be responsive to the analyte, the second section channel structure including a segment for delivering a substance to an analyzer of the substance, the substance in the segment including at least part of the analyte, the segment in the second section including an optically transparent region between an optical source and an optical detector of the analyzer.

21. The microchip of claim 20 wherein the transparent region is in a plane different from a plane including channel structures of the second portion.

22. A microfluidic microchip for chemical, physical, and/or biological analysis of an analyte comprising a substrate carrying a channel structure, the channel structure being arranged so the analyte can be moved to follow the channel structure by applying a potential to the channel structure, first and second portions of the channel structure being respectively parts of first and second sections of the substrate, the first section channel structure being deformable with respect to the second section channel structure, the first section channel structure being arranged to be responsive to the analyte, the second section channel structure including a segment for delivering a substance to an analyzer of the substance, the substance in the segment including at least part of the analyte, the segment for delivering at least one substance including a spray tip for spraying the substance into the analyzer.

23. The microchip of claim 22 wherein the channel structure of the first section and the segment for delivering are bent so they are in different planes, the spray tip having an axis in a plane different from the plane of the segment.

24. The microchip of claim 22 wherein the channel structure of the first section and the segment for delivering are bent so they are in the same plane as the spray tip having an axis in a plane different from the plane of the segment.

25. A microfluidic microchip for chemical, physical, and/or biological analysis of an analyte comprising a substrate carrying a channel structure, the channel structure being arranged so the analyte can be moved to follow the channel structure by applying a potential to the channel structure, first and second portions of the channel structure being respectively parts of first and second sections of the substrate, the first section channel structure being deformable with respect to the second section channel structure, the first section channel structure being arranged to be responsive to the analyte, the second section channel structure including a segment for delivering a substance to an analyzer of the substance, the substance in the segment including at least part of the analyte, the second section channel structure including a plurality of segments for selectively delivering to one substance sump the substance in said channel segment in the first portion, each of the plural segments of the second channel structure including a spray tip for spraying the substance in the particular segment into the analyzer.

26. A microfluidic microchip for chemical, physical, and/or biological analysis of an analyte comprising a substrate carrying a channel structure, the channel structure being arranged so the analyte can be moved to follow the channel structure by applying a potential to the channel structure, first and second portions of the channel structure being respectively parts of first and second sections of the substrate, the first section channel structure being deformable with respect to the second section channel structure, the first section channel structure-being arranged to be responsive to the analyte, the second section channel structure including a segment for delivering a substance to an analyzer of the substance, the substance in the segment including at least part of the analyte, the segment in the second section including an optically transparent region between an optical source and an optical detector of the analyzer, the transparent region being in a plane different from a plane including channel structures of the second portion.

27. The microchip of claim 26 wherein the potential is electrical.

28. The microchip of claim 26 wherein the potential is fluidic.