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DNA diagnostics based on mass spectrometry

  • US 6,602,662 B1
  • Filed: 11/28/2000
  • Issued: 08/05/2003
  • Est. Priority Date: 03/17/1995
  • Status: Expired due to Fees
First Claim
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1. A process for determining whether a target nucleotide is present in a nucleic acid molecule, comprising:

  • a) hybridizing a nucleic acid molecule with a primer oligonucleotide that is complementary to a sequence that is adjacent to a region suspected of containing the target nucleotide, thereby producing a hybridized primer;

    b) contacting the hybridized primer with i) deoxyribonucleoside triphosphates, ii) chain terminating nucleotides selected from the group consisting of dideoxyribonucleoside triphosphates and 3′

    -deoxynucleoside triphosphates, and iii) a DNA polymerase, whereby the hybridized primer is extended until a chain terminating nucleotide is incorporated, thereby producing an extended primer; and

    c) determining the molecular mass of the extended primer, thereby determining whether the target nucleotide is present in a nucleic acid molecule.

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