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Decoy probes

  • US 6,602,668 B2
  • Filed: 05/29/2001
  • Issued: 08/05/2003
  • Est. Priority Date: 07/31/1998
  • Status: Expired due to Fees
First Claim
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1. A purified decoy probe comprising:

  • a first nucleotide base recognition sequence region, wherein said first region binds to an RNA polymerase; and

    an optionally present second nucleotide base recognition sequence region, provided that if said first region is nucleic acid and said second region is present, then said second region is either directly joined to the 5′

    end of said first region is joined to the 3′

    end or 5′

    end of said first region by a non-nucleotide linker, wherein said optionally present second region is present if said first region can be used to produce a functional double-stranded promoter sequence using a complementary oligonucleotide, further provided that if said first region is nucleic acid which can be used to produce said functional double-stranded promoter sequence using said complementary oligonucleotide, then said decoy probe does not have a nucleic acid sequence greater than about 10 nucleotides in length joined directly to the 3′

    end of said first region and said decoy probe does not have a terminal 3′

    OH group available to accept a nucleoside triphosphate in a polymerization reaction.

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