Identification of compound-protein interactions using libraries of protein-nucleic acid fusion molecules
First Claim
1. A method for detecting interactions between one or more compounds and one or more protein-nucleic acid fusions, said method comprising:
- (a) providing a compound library which comprises a plurality of different compounds, wherein said compounds are immobilized on a solid support;
(b) simultaneously contacting said different immobilized compounds in a single reaction chamber with a protein-nucleic acid fusion library which comprises a plurality of different protein-nucleic acid fusions under conditions which allow the formation of one or more compound-fusion complexes;
(c) isolating said one or more immobilized compound-fusion complexes from unbound protein-nucleic acid fusions; and
(d) detecting said one or more compound-fusion complexes as an indication that one or more protein-nucleic acid fusions interact with one or more compounds.
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Abstract
Disclosed herein is a method for detecting a compound-protein interaction, involving: (a) providing a compound library in which each member of the compound library is immobilized on a solid support; (b) contacting each member of the immobilized compound library in a single reaction chamber with each member of a protein-nucleic acid fusion library under conditions which allow the formation of compound-fusion complexes; (c) isolating the immobilized compound-fusion complexes; and (d) detecting a compound-fusion complex as an indication that the protein of the fusion interacts with the compound. In preferred embodiments, the protein is identified by reading the nucleic acid portion of the fusion, and the compound is identified by reading a detectable tag bound to either the compound or the solid support.
75 Citations
32 Claims
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1. A method for detecting interactions between one or more compounds and one or more protein-nucleic acid fusions, said method comprising:
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(a) providing a compound library which comprises a plurality of different compounds, wherein said compounds are immobilized on a solid support;
(b) simultaneously contacting said different immobilized compounds in a single reaction chamber with a protein-nucleic acid fusion library which comprises a plurality of different protein-nucleic acid fusions under conditions which allow the formation of one or more compound-fusion complexes;
(c) isolating said one or more immobilized compound-fusion complexes from unbound protein-nucleic acid fusions; and
(d) detecting said one or more compound-fusion complexes as an indication that one or more protein-nucleic acid fusions interact with one or more compounds. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for detecting interactions between one or more compounds and one or more protein-nucleic acid fusions, said method comprising:
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(a) providing a compound library which comprises a plurality of different compounds, wherein said compounds are immobilized on a solid support;
(b) simultaneously contacting said library of different immobilized compounds with a protein-nucleic acid fusion library which comprises a plurality of different protein-nucleic acid fusions under conditions which allow said protein-nucleic acid fusions to bind to said compounds; and
(c) detecting one or more bound protein-nucleic acid fusions as an indication that one or more protein-nucleic acid fusions interact with one or more compounds of said compound library. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A method for detecting interactions between one or more compounds and one or more protein-nucleic acid fusions, said method comprising:
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(a) providing a compound library which comprises a plurality of compounds, wherein said compounds are immobilized on a plurality of support elements;
(b) contacting said immobilized compounds in a single reaction chamber with a protein-nucleic acid fusion library which comprises a plurality of protein-nucleic acid fusions under conditions which allow the formation of one or more compound-fusion complexes;
(c) sorting said one or more immobilized compound-fusion complexes on said support elements; and
(d) detecting said one or more compound-fusion complexes as an indication that one or more protein-nucleic acid fusions interact with one or more compounds of said compound library. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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Specification