Electric-field-assisted fluidic assembly of inorganic and organic materials, molecules and like small things including living cells

• US 6,605,453 B2
• Filed: 12/01/2000
• Issued: 08/12/2003
• Est. Priority Date: 12/01/1999
• Status: Expired due to Fees

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Bhatia, Sangeeta, Esener, Sadik, Ozkan, Mihrimah
• Primary Examiner(s): Weber, Jon P.
• Application Number:
  US09/728,801
• Publication Number:
  US 20020031813A1
• Time in Patent Office: 984 Days
• Field of Search: 435/173.1, 427/469, 204/484, 204/471
• US Class Current: 435/173.1
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00646 the compounds being bound to beads immobilised on the solid supports
• CPC Class Codes: B01J 2219/00653 the compounds being bound to electrodes embedded in or on the solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00743 Cells
• CPC Class Codes: C12N 15/1006 by means of a solid support carrier, e.g. particles, polymers

Abstract

Any of inorganic and bio-organic substances and molecules, and beads, pucks and like small things, that are both (i) electrically charged to a first polarity, and (ii) immersed in a fluid transport medium within (iii) an electrochemical cell, are assembled and patterned by action of moving these inorganic and bio-organic substances and molecules, etc. to a patterned electrode having an opposite, second, polarity under force of an applied electric field. The electrode patterned with conductive areas may be further, separately, patterned with chemicals, for example agarose gel, that chemically accept or reject the substances and molecules, etc., especially as are biological in origin. Living cells of plant, bacterial and animal types may be assembled. A semiconductor electrode may be patterned by masked laser light passed through the other electrode, which is transparent.

Claims

1. A method of assembling members of a group consisting of any of inorganic and bio-organic substances and molecules, and beads, pucks and other small things that are capable of holding an electrical charge, the method comprising:

photolithographically first-patterning a photosensitive electrode so that the electrode assumes first regions that are charged to a first electrical polarity and second regions that are charged to a second, opposite, electrical polarity; and

second-patterning members selected from the group consisting of any of inorganic and bio-organic substances and molecules, and beads, pucks and other small things that are both (i) electrically charged to a first polarity, and (ii) immersed in a fluid transport medium that is upon the electrode, by moving under force of an electric field these members to charged second regions of the first-patterned electrode which second regions are of an opposite electrical polarity to the members;

wherein the members respond to the electric field and accumulate on, and assume the pattern of, the second regions.

2. The assembly method according to claim 1

wherein the second-patterning is of group members that are negatively charged while the patterned electrode second regions are positively charged.

3. The assembly method according to claim 1

wherein the second-patterning is of group members that are positively charged while the patterned electrode second regions are negatively charged.

4. The assembly method according to claim 1

wherein the second-patterning is of members selected from the group consisting of bio-organic substances and molecules.

5. The assembly method according to claim 1

wherein the second-patterning is of members selected from the group consisting of biological cells and components of biological cells.

6. The assembly method according to claim 5

wherein the components of biological cells are selected from the group consisting of bio-organic substances and molecules obtained from biological cells.

7. The assembly method according to claim 1

wherein the second-patterning is of members selected from the group consisting of live biological cells.

8. The assembly method according to claim 7

wherein the live biological cells are selected from the group consisting of plant cells, bacterial cells and animal cells.

9. The assembly method according to claim 1

wherein the second-patterning is of members selected from the group consisting of biological molecules.

10. The assembly method according to claim 9

wherein the second-patterning is of DNA.

11. The assembly method according to claim 9

wherein the second-patterning is of members selected from the groups consisting of proteins.

12. The assembly method according to claim 1

wherein the fluid transport medium is bio-compatible with the living bio-organic substances and molecules.

13. The assembly method according to claim 1 further comprising, at a time and as a step before the photolithographically first-patterning, the additional step of:

coating the photosensitive electrode with a non-stick substance to which bio-organic substances, molecules and/or cells subsequently applied in the second-patterning step will not stick;

wherein the photolithographically first-patterning of the electrode also serves to pattern the non-stick substance.

14. The assembly method according to claim 13,

wherein the coating of the photosensitive electrode is with agarose gel.

15. The assembly method according to claim 1

wherein the photosensitive electrode consists essentially of a semiconductor.

16. A method of assembling any of inorganic and bio-organic substances and molecules, and beads, pucks and other small things that are capable of holding an electrical charge, the method comprising:

first patterning a photosensitive electrode by selectively exposing it with masked radiation so as to pattern the electrode into first and second electrically charged regions;

placing any of inorganic and bio-organic substances and molecules, and beads, pucks and other small things that are electrically charged to an electrical polarity opposite to that of the second charged region within a fluid transport medium upon the first-patterned photosensitive electrode; and

imposing an electrical field across the fluid transport medium so that the electrically-charged inorganic and bio-organic substances and molecules, and beads, pucks and other small things that are within the fluid transport medium will migrate to the second charged region of the patterned photosensitive electrode, which region is of opposite polarity to the polarity of the electrically-charged inorganic and bio-organic substances and molecules, and beads, pucks and other small things;

wherein the inorganic and bio-organic substances and molecules, and beads, pucks and other small things assume the pattern of the photosensitive electrode second region that resulted from exposure of the photosensitive electrode with masked radiation.

17. A method of assembling small things that are capable of holding an electrical charge, the method comprising:

spacing parallel a semiconductor first electrode at a separation from a transparent second electrode;

photolithographically patterning with light transmitted through the transparent second electrode the semiconductor first electrode so that first regions of the first electrode are electrically charged to a first electrical polarity while second regions of the first electrode are electrically charged to a second, opposite, electrical polarity;

placing a liquid medium containing small things that are capable of holding an electrical charge between the first and the second electrode; and

electrically charging the small things to a first electrical polarity;

wherein the small things that are electrically charged to the first electrical polarity are electrically attracted to the second regions of the patterned photosensitive electrode that are charged to the second electrical polarity, and will thus assume the pattern of these second regions.