Microfluidic devices with monolithic microwave integrated circuits

• US 6,605,454 B2
• Filed: 03/22/2001
• Issued: 08/12/2003
• Est. Priority Date: 09/16/1999
• Status: Expired due to Term

• Current Assignee: Google Technology Holdings LLC (Alphabet Inc.)
• Original Assignee: Motorola, Inc. (Motorola Solutions, Inc.)
• Inventors: Burdon, Jeremy, Rhine, David, Smekal, Thomas, Nair, Vijay, Grodzinski, Piotr, Dai, Xunhu, Gallagher, Sean, Marrero, Robert, Barenburg, Barbara Foley, Chan, Yuk-Tong
• Primary Examiner(s): Weber, Jon P.
• Application Number:
  US09/816,512
• Publication Number:
  US 20020115201A1
• Time in Patent Office: 873 Days
• Field of Search: 435/173.7, 219/690, 219/691, 219/692, 219/693, 422/22
• US Class Current: 435/173.7
• CPC Class Codes: C12N 1/066 by physical methods
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: H05B 6/806 for laboratory use

Abstract

A microwave device has a monolithic microwave integrated circuit (MMIC) disposed therein for applying microwave radiation to a microfluidic structure, such as a chamber, defined in the device. The microwave radiation from the MMIC is useful for heating samples introduced into the microfluidic structure and for effecting lysis of cells in the samples. Microfabrication techniques allow the fabrication of MMICs that perform heating and cell lysing of samples having volumes in the microliter to picoliter range.

Claims

1. A method for lysing cells in a cavity of a microfluidic device comprising:

introducing a cell into said cavity;

providing microwave radiation comprising at least one of a predetermined frequency, a predetermined wavelength and a predetermined intensity; and

heating said cavity for an effective duration of time with said microwave radiation from a monolithic microwave intograted circuit (MMIC) in order to form a cell lysate, wherein said MMIC is substantially integrated with said microfluidic device.

2. The method according to claim 1, wherein said MMIC comprises microstrip line conductors disposed substantially adjacent to said cavity.

3. The method according to claim 1, wherein said MMIC comprises slot line conductors disposed substantially adjacent to said cavity.

4. The method according to claim 1, wherein said cavity comprises at least one of a fluid chamber and a fluid channel.

5. The method according to claim 1, wherein said cell comprises at least one of a eukaryotic cell, a prokaryotic cell and a mammalian tumor cell.

6. The method according to claim 1, wherein said cavity comprises at least one of an inlet port and an outlet port, said inlet port substantially connected to a plurality of reagent sources;

and wherein said microfluidic device is operatively associated with a computer controller for controlling at least one of reagent flow and application of microwave radiation.

7. The method according to claim 6, wherein said MMIC is operatively associated with a microwave generator.

8. The method according to claim 6, wherein said reagent sources are substantially integrated into said microfluidic device.

9. The method according to claim 6, wherein said plurality of reagent sources comprise at least one of lysis buffer, magnetic nucleic acid absorption bead solution, high salt buffer and low salt buffer.

10. A method for lysing viral particles in a cavity of a microfluidic device comprising:

introducing a viral particle into said cavity;

providing microwave radiation comprising at least one of a predetermined frequency, a predetermined wavelength and a predetermined intensity; and

heating said cavity for an effective duration of time with said microwave radiation from a monolithic microwave integrated circuit (MMIC) in order to form a viral particle lysate, wherein said MMIC is substantially integrated with said microfluidic device.