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Method for high-density microarray medicated gene expression profiling

  • US 6,607,885 B1
  • Filed: 10/11/2000
  • Issued: 08/19/2003
  • Est. Priority Date: 10/15/1999
  • Status: Expired due to Fees
First Claim
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1. A method for identifying gene expression changes within a bacterial species comprising:

  • (a) providing a comprehensive micro-array synthesized from DNA comprised in a bacterial species;

    (b) generating a first set of labeled probes from bacterial RNA, the RNA isolated from the bacterial species of step (a);

    (c) hybridizing the first set of labeled probes of step (b) to the comprehensive micro-array of step (a), wherein hybridization results in a detectable signal generated from the labeled probe;

    (d) measuring the signal generated by the hybridization of the first set of labeled probe to the comprehensive micro-array of step (c);

    (e) subjecting the bacterial species of step (a) to a gene expression altering condition whereby the gene expression profile of the bacterial species is altered to produce a modified bacterial species;

    (f) generating a second set of labeled probes from bacterial RNA, the RNA isolated from the modified bacterial species of step (e);

    (g) hybridizing the second set of labeled probes of step (f) to the comprehensive micro-array of step (a), wherein hybridization results in a detectable signal generated from the labeled probe;

    (h) measuring the signal generated by the hybridization of the second set of labeled probes to the comprehensive micro-array of step (g); and

    (i) comparing signal generated from the first hybridization to the signal generated from the second hybridization to identify gene expression changes within a bacterial species.

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