Method of promoting chondrocyte differentiation with hedgehog related polypeptides
First Claim
1. A method for promoting differentiation of a chondrocyte, comprising contacting said chondrocyte with an effective amount of a polypeptide, wherein the polypeptide includes a hedgehog polypeptide encoded by a nucleic acid which hybridizes under stringent conditions, including a wash step of 0.2×
- SSC at 65°
C., to at least one of SEQ ID NO;
1, SEQ ID NO;
4, SEQ ID NO;
5, or SEQ ID NO;
6, and which hedgehog polypeptide binds a patched polypeptide and promotes hedgehog signal transduction.
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Accused Products
Abstract
The present invention concerns the discovery that proteins encoded by a family of vertebrate genes, termed here hedgehog-related genes, comprise morphogenic signals produced by embryonic patterning centers, and are involved in the formation of ordered spatial arrangements of differentiated tissues in vertebrates. The present invention makes available compositions and methods that can be utilized, for example to generate and/or maintain an array of different vertebrate tissue both in vitro and in vivo.
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Citations
27 Claims
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1. A method for promoting differentiation of a chondrocyte, comprising contacting said chondrocyte with an effective amount of a polypeptide, wherein the polypeptide includes a hedgehog polypeptide encoded by a nucleic acid which hybridizes under stringent conditions, including a wash step of 0.2×
- SSC at 65°
C., to at least one of SEQ ID NO;
1, SEQ ID NO;
4, SEQ ID NO;
5, or SEQ ID NO;
6, and which hedgehog polypeptide binds a patched polypeptide and promotes hedgehog signal transduction. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 21, 22, 23, 24, 25, 26, 27)
- SSC at 65°
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18. A method for promoting differentiation of mammalian chondrocytes responsive to hedgehog induction, comprising treating the chondrocytes with an amount of a polypeptide effective to promote the differentiation of the chondrocytes, wherein the polypeptide includes a hedgehog polypeptide greater than 90% identical to at least one of SEQ ID NO:
- 8, SEQ ID NO;
11, SEQ ID NO;
12, or SEQ ID NO;
13, and which hedgehog polypeptide binds a patched polypeptide and promotes hedgehog signal transduction. - View Dependent Claims (19, 20)
- 8, SEQ ID NO;
Specification